Abstract：
:Clostridium saccharoperbutylacetonicum N1-4 is well known as a hyper-butanol producing strain. However, little information is available concerning its butanol production mechanism and the development of more robust strains. In this study, key biosynthetic genes (either endogenous or exogenous) including the sol operon (bld-ctfA-ctfB-adc), adhE1, adhE1D485G, thl, thlA1V5A, thlAV5A and the expression cassette EC (thl-hbd-crt-bcd) were overexpressed in C. saccharoperbutylacetonicum N1-4 to evaluate their potential in enhancement of butanol production. The overexpression of sol operon increased ethanol production by 400%. The overexpression of adhE1 and adhED485G resulted in a 5.6- and 4.9-fold higher ethanol production, respectively, producing final acetone-butanol-ethanol (ABE) titers (30.6 and 30.1gL-1) of among the highest as ever reported for solventogenic clostridia. The most significant increase of butanol production (by 13.7%) and selectivity (73.7%) was achieved by the overexpression of EC. These results provides a solid foundation and essential references for the further development of more robust strains.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Wang S,Dong S,Wang Ydoi
10.1016/j.biortech.2017.09.024subject
Has Abstractpub_date
2017-12-01 00:00:00pages
426-433issue
Pt Aeissn
0960-8524issn
1873-2976pii
S0960-8524(17)31572-9journal_volume
245pub_type
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