Abstract：
:To achieve a sustainable production of food and feed production, inexpensive carbon and nutrient sources are needed. In the present study, biologically upgraded biogas is coupled with electrochemically extracted nitrogen from digested biowaste to cultivate mixed methanotrophs as protein source. Results showed that an increase from less than 5 μgCu2+/L to 100 μgCu2+/L increased the biomass production by 41%. Microbial analysis revealed that the dominated Methylomonas spp. followed by Methylophilus spp. created a specialized community for high CH4 assimilation. Moreover, duplicate semi-continuous fermenters run for 120 days validating the efficiency of alternative carbon and nitrogen feedstocks at long-term operation. As for dry cell weight (DCW) production, more than 2.5 g-DCW/L were produced using biologically upgraded biogas and electrochemically extracted nitrogen. Furthermore, the protein content and amino acid profile (>50% of DCW) demonstrated that the microbial biomass pose the characteristics to be used as animal feed additive.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Tsapekos P,Zhu X,Pallis E,Angelidaki Idoi
10.1016/j.biortech.2020.123646subject
Has Abstractpub_date
2020-10-01 00:00:00pages
123646eissn
0960-8524issn
1873-2976pii
S0960-8524(20)30918-4journal_volume
313pub_type
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