Abstract：
:The aim of this work was to study the accumulation of phytoene in Dunaliella salina V-101 by down-regulating its phytoene desaturase (PDS) gene expression using RNA interference and Antisense technology. RNAi and antisense constructs were introduced into the Dunaliella cells by Agrobacterium-mediated transformation. Among thirty-two transformants, six showed positive down-regulation of PDS expression with RNAi construct and five positive transformants were obtained using antisense construct. Characterization of PDS suppression was carried out using semi-quantitative RT-PCR and quantitative determination of phytoene as well as other carotenoids by HPLC. Both the RNAi and antisense lines showed a significant decrease in the expression levels of phytoene desaturase and carotenoid content compared to wild type cells. The RNAi line #5 showed maximum Phytoene content (108.34±22.34µg/100mg DCW) compared to other transgenic lines. These phytoene-accumulating phenotypes exhibited slower growth rates and were found to be sensitive to high light conditions.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Srinivasan R,Babu S,Gothandam KMdoi
10.1016/j.biortech.2017.03.042subject
Has Abstractpub_date
2017-10-01 00:00:00pages
311-318eissn
0960-8524issn
1873-2976pii
S0960-8524(17)30312-7journal_volume
242pub_type
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