Abstract：
:This work aimed to select a Tisochrysis lutea phenotype with higher biomass and fucoxanthin productivities using fluorescence-activated cell sorting (FACS). A novel phenotype was obtained after 2 rounds of selection, based on high-fucoxanthin fluorescence. The resulting phenotype forms cell aggregates, has no flagella, and was stable after 15 months. Optimal temperature (30 °C) and light (300 µmol m-2 s-1) were obtained at laboratory scale, identical to the original strain. The biomass productivity was higher than the original strain: 1.9× at laboratory scale (0.4 L), and 4.5× under outdoor conditions (190 L). Moreover, compared to the original strain, the productivity of fucoxanthin increased 1.6-3.1× and docosahexaenoic acid 1.5-1.9×. These are the highest ever reported outdoor productivities, obtained with a robust new phenotype from a T. lutea monoculture isolated with FACS without genetic manipulation. The resulting phenotype shows high potential for industrial production.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Gao F,Sá M,Cabanelas ITD,Wijffels RH,Barbosa MJdoi
10.1016/j.biortech.2021.124725subject
Has Abstractpub_date
2021-01-18 00:00:00pages
124725eissn
0960-8524issn
1873-2976pii
S0960-8524(21)00064-Xjournal_volume
325pub_type
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