Reducing cofactors contribute to the increase of butanol production by a wild-type Clostridium sp. strain BOH3.

Abstract:

:Availability of reducing factors (e.g., NADH and NADPH) plays an important role in improving the efficacy of products conversion in cofactor-dependent production systems. In this study, nicotinic acid (NA), the precursor of NADH and NADPH, was supplemented to the growth medium of a wild-type Clostridium sp. strain BOH3. Results showed that the addition of precursor NA to the medium led to a significant increase in the levels of NADH and NADPH. Meanwhile, a maximal cell growth rate and butanol generation rate were reached by applying a two-stage pH-shift strategy, achieving 18.7g/L butanol with a yield of 24.6% and a productivity of 0.26g/Lh. The metabolic patterns were shifted towards more reduced metabolites as reflected by higher butanol-to-acetone ratio (11%) and butanol-to-acid ratio (292%). Redistributing metabolic flux to butanol via manipulations of reducing cofactor and pH shift could become an alternative tool to realize metabolic engineering goals.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Li T,Yan Y,He J

doi

10.1016/j.biortech.2013.12.089

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

220-8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(13)01929-9

journal_volume

155

pub_type

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