The use of NaCl addition for the improvement of polyhydroxyalkanoate production by Cupriavidus necator.

Abstract:

:External stress factors in the form of ionic species or temperature increases have been shown to produce a stress response leading to enhanced PHA production. The effect of five different NaCl concentrations, namely 3.5, 6.5, 9, 12 and 15 g/l NaCl on PHA productivity using Cupriavidus necator has been investigated alongside a control (no added NaCl). A dielectric spectroscopy probe was used to measure PHA accumulation online in conjunction with the chemical offline analysis of PHA. The highest PHA production was obtained with the addition of 9 g/l NaCl, which yielded 30% higher PHA than the control. Increasing the addition of NaCl to 15 g/l was found to inhibit the production of PHA. NaCl addition can therefore be used as a simple, low cost, sustainable, non toxic and non reactive external stress strategy for increasing PHA productivity.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Passanha P,Kedia G,Dinsdale RM,Guwy AJ,Esteves SR

doi

10.1016/j.biortech.2014.04.068

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

287-94

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)00608-7

journal_volume

163

pub_type

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