Use of a triiodide resin for isolation of axenic cultures of microalgal Nannochloropsis gaditana.

Abstract:

:Triiodide resin (TR) was used to generate axenic cultures of microalgae by employing the antibacterial capability of triiodide. A Nannochloropsis gaditana culture contaminated with bacteria was passed through a column filled with TR using the gravity flow. Based on analyses of flow cytometry and vital staining using a fluorescent dye SYTOX Green, three cycles of TR treatments remarkably reduced the number of viable bacteria but had little effects on the microalgae. This novel approach is a simple, rapid, and cost-effective method that can be used to isolate axenic cultures of microalgae.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Nam K,Shin WS,Jeong BR,Park MS,Yang JW,Kwon JH

doi

10.1016/j.biortech.2015.03.082

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

391-4

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(15)00414-9

journal_volume

191

pub_type

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