Abstract:
:Constructs carrying the Saccharomycopsis fibuligera β-glucosidase gene (BGL1) under the control of a constitutive actin or a galactose-inducible promoter were introduced into eleven Saccharomyces strains. In ten of these recombinant strains, BGL1 expression driven by the actin promoter was between 1.6- and 18-fold higher than that obtained with the galactose-inducible promoter. Strains carrying the actin promoter yielded ethanol concentrations from cellobiose of between 0.5% and 14%, depending on their ability to accumulate Bgl1 (between 30 and 250 mU/mL) but also on their genetic background. Comparative analysis of a S. cerevisiae strain and its corresponding petite version showed similar ethanol yields, despite a 3-fold lower β-glucosidase production of the latter, suggesting that respiratory activity could be one of the factors influencing ethanol production when using carbon sources other than glucose. This study provides a selection of strains that may be good candidates as hosts for ethanol biosynthesis from cellulosic substrates.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Gurgu L,Lafraya Á,Polaina J,Marín-Navarro Jdoi
10.1016/j.biortech.2011.01.062subject
Has Abstractpub_date
2011-04-01 00:00:00pages
5229-36issue
8eissn
0960-8524issn
1873-2976pii
S0960-8524(11)00133-7journal_volume
102pub_type
杂志文章abstract::Microalgae contain about 50% of carbon, which means that a total of 1.83 ton of CO2 is needed to produce 1 ton of microalgae. The cost of CO2 supply for microalgal large scale cultivation should be considered and the low CO2 fixation efficiency by microalgae will lead to much more expenditure of CO2. In this study, a ...
journal_title:Bioresource technology
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2017.12.013
更新日期:2018-02-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:2013-07-01 00:00:00
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