Fermentation of cellobiose to ethanol by industrial Saccharomyces strains carrying the β-glucosidase gene (BGL1) from Saccharomycopsis fibuligera.

Abstract:

:Constructs carrying the Saccharomycopsis fibuligera β-glucosidase gene (BGL1) under the control of a constitutive actin or a galactose-inducible promoter were introduced into eleven Saccharomyces strains. In ten of these recombinant strains, BGL1 expression driven by the actin promoter was between 1.6- and 18-fold higher than that obtained with the galactose-inducible promoter. Strains carrying the actin promoter yielded ethanol concentrations from cellobiose of between 0.5% and 14%, depending on their ability to accumulate Bgl1 (between 30 and 250 mU/mL) but also on their genetic background. Comparative analysis of a S. cerevisiae strain and its corresponding petite version showed similar ethanol yields, despite a 3-fold lower β-glucosidase production of the latter, suggesting that respiratory activity could be one of the factors influencing ethanol production when using carbon sources other than glucose. This study provides a selection of strains that may be good candidates as hosts for ethanol biosynthesis from cellulosic substrates.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Gurgu L,Lafraya Á,Polaina J,Marín-Navarro J

doi

10.1016/j.biortech.2011.01.062

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

5229-36

issue

8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)00133-7

journal_volume

102

pub_type

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