Abstract：
:Physical adsorption preserving activity and support reusability was used to directly bond lipase from Thermomyces lanuginosus on citric acid (CA) modified Fe3O4/Au magnetic nanoparticles. A new faster approach has been used for CA ligand exchange, which ensures an high payload of stable enzyme. The immobilized lipase was tested for the biodiesel production from spent coffee ground in a solvent free system. It retains, after 60 days, more than 90% of its initial activity. Biodiesel yield of 51.7%, after 3 h of synthesis, which increases up to ∼100% after 24 h indicating an enzymatic fast kinetic, was measured. No significant decrease, during the first three cycles of use, of the lipase activity occurs. The biodiesel presents an ester content of 98.4 ± 0.23 in agreement with the EN14214. The iodine value of 76.67 (g iodine/100 g) is in agreement with the European standard.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Sarno M,Iuliano Mdoi
10.1016/j.biortech.2018.06.108subject
Has Abstractpub_date
2018-10-01 00:00:00pages
431-438eissn
0960-8524issn
1873-2976pii
S0960-8524(18)30887-3journal_volume
266pub_type
杂志文章abstract：:The aim of this study was to evaluate the potential of Hancornia speciosa GOMES (mangaba) seeds as a novel matrix for the production of bio-oil. The study was divided into three steps: (i) characterization of the biomass (through elemental analysis (CHN), infrared spectroscopy (FTIR-ATR), thermogravimetry (TG), and de...
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