Immobilization of penicillin G acylase on macro-mesoporous silica spheres.

Abstract:

:In this study, macro-mesoporous silica spheres were prepared with a micro-device and used as the support for the immobilization of penicillin G acylase (PGA). To measure the enzymatic activity, the silica spheres with immobilized PGA were placed into a packed-bed reactor, in which the hydrolysis of penicillin G was carried out. The influences of the residence time, the initial concentration of the substrate, the accumulation of the target product 6-aminopenicillanic acid, and the enzyme loading amount on the performance of the immobilized PGA were investigated. The introduction of macropores increased the enzyme loading amount and decreased the internal mass transfer resistance, and the results showed that the enzyme loading amount reached 895 mg/g (dry support), and the apparent enzymatic activity achieved up to 1033 U/g (dry support). In addition, the immobilized PGA was found to have great stability.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Zhao J,Wang Y,Luo G,Zhu S

doi

10.1016/j.biortech.2010.09.076

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

529-35

issue

2

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)01608-1

journal_volume

102

pub_type

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