Biodegradation of lignin by fungi, bacteria and laccases.

Abstract:

:Indulin AT biodegradation by basidiomycetous fungi, actinobacteria and commercial laccases was evaluated using a suite of chemical analysis methods. The extent of microbial degradation was confirmed by novel thermal carbon analysis (TCA), as the treatments altered the carbon desorption and pyrolysis temperature profiles in supernatants. Laccase treatments caused only minor changes, though with increases occurring in the 850°C and char precursor fractions. After fungal treatments, lignin showed a similar change in the TCA profile, along with a gradual decrease of the total carbon, signifying lignin mineralization (combined with polymerization). By contrast, bacteria produced phenolic monomers without their further catabolism. After 54days of cultivation, a 20wt% weight loss was observed only for fungi, Coriolus versicolor, corroborating the near-80% carbon mass balance closure obtained by TCA. Compositional changes in lignin as a result of biodegradation were confirmed by thermal desorption (TD)-pyrolysis-GC-MS validating the carbon fractionation obtained by TCA.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Asina F,Brzonova I,Voeller K,Kozliak E,Kubátová A,Yao B,Ji Y

doi

10.1016/j.biortech.2016.08.016

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

414-424

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(16)31137-3

journal_volume

220

pub_type

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