当前位置： SCI文献检索 > BIORESOURCE TECHNOLOGY期刊下所有文献 > Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3.

Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3.

Abstract：

:Lactobacillus brevis OPK-3, having 84.292 mg/L/h of gamma-aminobutyric acid (GABA) productivity, was isolated from Kimchi, a traditional fermented food in Korea. A core fragment of glutamate decarboxylase (GAD) DNA was isolated from the L. brevis OPK-3, using primers based on two highly conserved regions of GAD. A full-length GAD (LbGAD) clone was subsequently isolated through rapid amplification of cDNA ends (RACE) PCR. Nucleotide sequence analysis revealed that the open reading frame (ORF) consisted of 1401 bases and encoded a protein of 467 amino acid residues with a calculated molecular weight of 53.4 kDa and a pI of 5.65. The amino acid sequence deduced from LbGAD ORF showed 83%, 71%, and 60% identity to the Lactobacillus plantarum GAD, Lactococcus lactis GAD, and Listeria monocytogenes GAD sequences, respectively. The LbGAD gene was expressed in Escherichia coli strain UT481, and the extract of transformed E. coli UT481 contained an induced 53.4 kDa protein and had significantly enhanced GAD activity.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Park KB,Oh SH

doi

10.1016/j.biortech.2006.01.004

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

312-9

issue

2

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(06)00021-6

journal_volume

98

pub_type

杂志文章

相关文献

BIORESOURCE TECHNOLOGY文献大全
  • Lipid production for second generation biodiesel by the oleaginous yeast Rhodotorula graminis.

    abstract：:The increasing cost of vegetable oils is turning the use of microbial lipids into a competitive alternative for the production of biodiesel fuel. The oleaginous yeast Rhodotorula graminis is able to use a broad range of carbon sources for lipid production, and is able to resist some of the inhibitors commonly released...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.02.004

    authors： Galafassi S,Cucchetti D,Pizza F,Franzosi G,Bianchi D,Compagno C

    更新日期：2012-05-01 00:00:00

  • Enhanced dewatering of excess activated sludge through decomposing its extracellular polymeric substances by a Fe@Fe2O3-based composite conditioner.

    abstract：:Efficient sludge dewatering methods are highly desired by municipal wastewater treatment plants. In this study, Fe@Fe2O3 nanomaterial, combined with polydiallyldimethylammonium chloride (PDMDAAC) and H2SO4, was used for sludge dewatering. This composite conditioner exhibited an excellent dewatering capability. By usin...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2016.06.139

    authors： He DQ,Luo HW,Huang BC,Qian C,Yu HQ

    更新日期：2016-10-01 00:00:00

  • Improvement of gaseous energy recovery from sugarcane bagasse by dark fermentation followed by biomethanation process.

    abstract：:The aim of the present study was to enhance the gaseous energy recovery from sugarcane bagasse. The two stage (biohydrogen and biomethanation) batch process was considered under mesophilic condition. Alkali pretreatment (ALP) was used to remove lignin from sugarcane bagasse. This enhanced the enzymatic digestibility o...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2015.07.038

    authors： Kumari S,Das D

    更新日期：2015-10-01 00:00:00

  • Evaluation of different water-washing treatments effects on wheat straw combustion properties.

    abstract：:A series of experiments was conducted to explore the effects of various water-washing solid-liquid ratios (1:50 and 1:10) and the stirring on wheat straw (WS) combustion properties. Comparing different solid-liquid ratio groups, a 16% increment in the higher heating value was obtained for 1:50 groups and only 5% for 1...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.09.052

    authors： Ma Q,Han L,Huang G

    更新日期：2017-12-01 00:00:00

  • Experiences with the dual digestion of municipal sewage sludge.

    abstract：:The dual digestion process was investigated using sludge samples collected at the WWTP of Tomaszow Mazowiecki (Poland). Mixed sludge was treated in a laboratory setup under batch and semi-continuous conditions. Dual digestion with a 1d SRT aerobic thermophilic pretreatment followed by an anaerobic step with 20 d of SR...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2006.05.017

    authors： Borowski S,Szopa JS

    更新日期：2007-04-01 00:00:00

  • New functional biocarriers for enhancing the performance of a hybrid moving bed biofilm reactor-membrane bioreactor system.

    abstract：:In this study, new sponge modified plastic carriers for moving bed biofilm reactor (MBBR) was developed. The performance and membrane fouling behavior of a hybrid MBBR-membrane bioreactor (MBBR-MBR) system were also evaluated. Comparing to the MBBR with plastic carriers (MBBR), the MBBR with sponge modified biocarrier...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2016.02.057

    authors： Deng L,Guo W,Ngo HH,Zhang X,Wang XC,Zhang Q,Chen R

    更新日期：2016-05-01 00:00:00

  • Optimization of biodiesel supply chains based on small farmers: a case study in Brazil.

    abstract：:This article presents a methodology for conceiving and planning the development of an optimized supply chain of a biodiesel plant sourced from family farms and taking into consideration agricultural, logistic, industrial, and social aspects. This model was successfully applied to the production chain of biodiesel fuel...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2011.07.002

    authors： Leão RR,Hamacher S,Oliveira F

    更新日期：2011-10-01 00:00:00

  • Comparison of catalytically non-productive adsorption of fungal proteins to lignins and pseudo-lignin using isobaric mass tagging.

    abstract：:Catalytically non-productive adsorption of fungal enzymes to pseudo-lignin (PL) was compared to adsorption to lignin preparations derived from different sources (SL, spruce; BL, birch; OL, beech) using different methods [steam pretreatment/enzymatic saccharification (SL, BL) and organosolv processing (OL)]. The protei...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2018.07.149

    authors： Wang Z,Jönsson LJ

    更新日期：2018-11-01 00:00:00

  • Symbiotic relationship between hydrothermal carbonization technology and anaerobic digestion for food waste in China.

    abstract：:Food waste (FW) is traditionally disposed through landfills and incineration in China. Nowadays, there are some promising methods, such as anaerobic digestion (AD) or feeding and composting, which are being applied in pilot cities. However, the inherent characteristics of Chinese FW may be regarded as a double-edged s...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/j.biortech.2018.03.102

    authors： Zhou Y,Engler N,Nelles M

    更新日期：2018-07-01 00:00:00

  • Enhanced solubilization and biochemical methane potential of waste activated sludge by combined free nitrous acid and potassium ferrate pretreatment.

    abstract：:The increasing production of waste activated sludge (WAS) from wastewater treatment plants presents an inherent environmental burden. In this study, Free nitrous acid combined with potassium ferrate (FNA + PF) pretreatment was used to enhance solubilization and biochemical methane potential of WAS. Results indicated t...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.122376

    authors： Wang J,Zhang Z,Ye X,Pan X,Lv N,Fang H,Chen S

    更新日期：2020-02-01 00:00:00

  • Microwave-assisted hydrolysis of Zymomonas mobilis levan envisaging oligofructan production.

    abstract：:Levan, a polysaccharide from Zymomonas mobilis, was hydrolyzed to obtain oligofructans or fructooligosaccharides with a degree of polymerization varying from 4 to 14. Fructooligosaccharides (FOS) are short chain fructans that beneficially affects the host by selective stimulation of growth and activity of one or a num...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2007.04.062

    authors： de Paula VC,Pinheiro IO,Lopes CE,Calazans GC

    更新日期：2008-05-01 00:00:00

  • Characterization of a protease-resistant α-galactosidase from the thermophilic fungus Rhizomucor miehei and its application in removal of raffinose family oligosaccharides.

    abstract：:The α-galactosidase gene, RmGal36, from Rhizomucor miehei was cloned and expressed in Escherichia coli. The gene has an open reading frame of 2256bp encoding 751 amino acid residues. RmGal36 was optimally active at pH 4.5 and 60°C, but is stable between pH 4.5 and 10.0 and at a temperature of up to 55°C for 30min reta...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.01.144

    authors： Katrolia P,Jia H,Yan Q,Song S,Jiang Z,Xu H

    更新日期：2012-04-01 00:00:00

  • Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria.

    abstract：:In this study, we report the ability of Stenotrophomonas maltophilia, Ochrobactrum anthropi, Pseudomonas mendocina, Microbacterium esteraromaticum and Pseudomonas aeruginosa to degrade multiple polycyclic aromatic hydrocarbons (PAHs) present in crude oil. The PAHs in the crude oil sample obtained from Digboi oil refin...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2018.01.075

    authors： Kumari S,Regar RK,Manickam N

    更新日期：2018-04-01 00:00:00

  • Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply.

    abstract：:Arthrospira (Spirulina) platensis, a fast-growing halophilic cyanobacterium able to accumulate glycogen, was investigated for its feasibility to serve as feedstock for fermentative production of biofuels and chemicals. The culture conditions most appropriate for glycogen production were identified. Glycogen production...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.01.004

    authors： Aikawa S,Izumi Y,Matsuda F,Hasunuma T,Chang JS,Kondo A

    更新日期：2012-03-01 00:00:00

  • Optimization of fed-batch enzymatic hydrolysis from alkali-pretreated sugarcane bagasse for high-concentration sugar production.

    abstract：:Fed-batch enzymatic hydrolysis process from alkali-pretreated sugarcane bagasse was investigated to increase solids loading, produce high-concentration fermentable sugar and finally to reduce the cost of the production process. The optimal initial solids loading, feeding time and quantities were examined. The hydrolys...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2014.05.034

    authors： Gao Y,Xu J,Yuan Z,Zhang Y,Liu Y,Liang C

    更新日期：2014-09-01 00:00:00

  • Efficient removal of heavy metals by synergistic actions of microorganisms and waste molasses.

    abstract：:In this study, two bacteria strains (Enterobacter sp. SL and Acinetobacter sp. SL-1) and waste molasses (carbon source) were used to remove Zn(II), Cd(II), Cr(VI), and Cr(Total) in the liquid solution (87 mg·L). The results showed the removal efficiencies of Cr(Total) and Cr(VI) could reach over 98.00% after reaction,...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2020.122797

    authors： Sun Y,Lan J,Du Y,Li Z,Liao X,Du D,Ye H,Zhang TC,Chen S

    更新日期：2020-04-01 00:00:00

  • Biological removal of nitrogen oxides by microalgae, a promising strategy from nitrogen oxides to protein production.

    abstract：:Nitrogen oxides (NOx) are the components of fossil flue gases that give rise to serious environmental and health hazards. Among the available techniques for NOx removal, microalgae-based biological removal of NOx (BioDeNOx) is a promising and competent technology with eco-friendly path of low energy and low-cost solut...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/j.biortech.2019.122037

    authors： Qie F,Zhu J,Rong J,Zong B

    更新日期：2019-11-01 00:00:00

  • Impact of worm predation on pseudo-steady-state of the circulating fluidized bed biofilm reactor.

    abstract：:This paper studies integrated simultaneous carbon and nitrogen removal as well as worm predation, in a circulating fluidized bed biofilm reactor (CFBBR) operated with an anoxic-aerobic bioparticle recirculation. A lab-scale CFBBR with a 8.5-liter reaction zone comprising 2L anoxic and 6.5L aerobic compartments was des...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.10.069

    authors： Li M,Nakhla G,Zhu J

    更新日期：2013-01-01 00:00:00

  • Alkalinity and salinity favor bioelectricity generation potential of Clostridium, Tetrathiobacter and Desulfovibrio consortium in Microbial Fuel Cells (MFC) treating sulfate-laden wastewater.

    abstract：:Clostridium, Tetrathiobacter and Desulfovibrio species are identified as suitable biocatalysts for treating organic-rich and sulfate-laden wastewater. Results from this study show that the power generation was much higher under alkaline conditions, i.e., pH of 8 when compared to neutral and acidic conditions. The effe...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2020.123110

    authors： Kumar SS,Kumar V,Gnaneswar Gude V,Malyan SK,Pugazhendhi A

    更新日期：2020-03-02 00:00:00

  • The effectiveness of lime, chicken manure and leaf litter ash in ameliorating acidity in a soil previously under black wattle (Acacia mearnsii) plantation.

    abstract：:There are about 130,000 hectares of land in South Africa that have been under black wattle plantation for a long time and whose soils have become more acidic than those from contiguous land without the tree. This incubation study investigated the effectiveness of lime, chicken manure and leaf litter ash to ameliorate ...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/s0960-8524(02)00065-2

    authors： Materechera SA,Mkhabela TS

    更新日期：2002-10-01 00:00:00

  • LLE experimental data, thermodynamic modeling and sensitivity analysis in the ethyl biodiesel from macauba pulp oil settling step.

    abstract：:The aim of this study was to obtain experimental data related to liquid–liquid equilibrium (LLE) of systems containing glycerol + ethanol + ethyl biodiesel from macauba pulp oil, perform thermodynamic modeling and simulate the settling step of this biodiesel using simulation software. Binary interaction parameters wer...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.12.190

    authors： Basso RC,da Silva CA,Sousa Cde O,Meirelles AJ,Batista EA

    更新日期：2013-03-01 00:00:00

  • Chelating and radical scavenging activities of soy protein hydrolysates prepared from microbial proteases and their effect on meat lipid peroxidation.

    abstract：:Three commercial microbial proteases, neutral protease from Bacillus subtilis (NP), validase from Aspergillus oryze (Val), and alkaline protease from Bacillus licheniformis (AP), were investigated for producing antioxidant hydrolysates from soy protein. The resulting hydrolysates were fractioned by sequential ultrafil...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2009.11.078

    authors： Zhang L,Li J,Zhou K

    更新日期：2010-04-01 00:00:00

  • Comparison of deep eutectic solvents (DES) on pretreatment of oil palm empty fruit bunch (OPEFB): Cellulose digestibility, structural and morphology changes.

    abstract：:In this work, a novel solvent, deep eutectic solvent (DES) was applied to examine its effectiveness in pretreating OPEFB. Three types of DESs, i.e. choline chloride-lactic acid (ChCl-LA), choline chloride-urea (ChCl-U) and choline chloride-glycerol (ChCl-G) were investigated. The pretreatment performance was based on ...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.03.065

    authors： Thi S,Lee KM

    更新日期：2019-06-01 00:00:00

  • Statistical optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16.

    abstract：:The optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16 was conducted using statistical experimental design and analysis. Plackett-Burman design was first used to screen the most important variables influencing hydrogen production, and subsequently cent...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2009.11.031

    authors： Cao GL,Ren NQ,Wang AJ,Guo WQ,Yao J,Feng YJ,Zhao QL

    更新日期：2010-03-01 00:00:00

  • Analysis of enzymolysis process kinetics and estimation of the resource conversion efficiency to corn cobs with alkali soaking, water and acid steam explosion pretreatments.

    abstract：:This short communication analyzed enzymolysis kinetics and estimated the resource conversion efficiency of corn cobs. Three pretreatments were applied, and enzymatic hydrolysis was performed to obtain solid residues. The enzymolysis process was fit to a modified logistic formula. The kinetics of enzymolysis were simil...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2018.06.045

    authors： Han Y,Xu J,Zhao Z,Zhao J

    更新日期：2018-09-01 00:00:00

  • Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae.

    abstract：:In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endo...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2008.11.047

    authors： Matsushika A,Inoue H,Murakami K,Takimura O,Sawayama S

    更新日期：2009-04-01 00:00:00

  • Management of root lesion nematode, Pratylenchus delattrei in crossandra using oil cakes.

    abstract：:Selected oil cakes, neem, castor and mahua, were tried independently and in combination with a chemical nematicide (carbofuran 3G) for the management of Pratylenchus delattrei in crossandra under glass house conditions. The neem oil cake was effective compared to other oil cakes used and there was a synergistic effect...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2003.11.002

    authors： Jothi G,Babu RS,Ramakrishnan S,Rajendran G

    更新日期：2004-07-01 00:00:00

  • Slow pyrolysis of rice straw: analysis of products properties, carbon and energy yields.

    abstract：:Among many uses of rice straw, application of its biochar from pyrolysis to the soil is receiving greater interest for increased crop productivity and sequestration of CO2. This study investigated slow pyrolysis of rice straw at 300-700°C to characterize the yields and detailed composition of the biochar, bio-oil and ...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2013.12.084

    authors： Park J,Lee Y,Ryu C,Park YK

    更新日期：2014-03-01 00:00:00

  • Using poly([1-vinyl-3-hexylimidazolium] [bis(trifluoromethylsulfonyl)imide]) to adsorb bio-ethanol from a Chamaecyparis obtuse leaves fermentation broth.

    abstract：:Poly([1-vinyl-3-hexylimidazolium] [bis(trifluoromethylsulfonyl)imide]) (poly([VHIM][Tf2N])) was assessed for its ability to adsorb bio-ethanol from Chamaecyparis obtuse leaves fermentation broths. Poly([VHIM][Tf2N]) was prepared by poly([VHIM][Br]) ion exchange with Li(Tf2N). Poly([VHIM][Br]) was obtained using a ther...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2013.03.113

    authors： Tang B,Bi W,Row KH

    更新日期：2013-06-01 00:00:00

  • Using cavitation for delignification of wood.

    abstract：:The Kraft process is the most widely used chemical process for the removal of lignin and other polymers from wood to obtain cellulosic pulp fibres. In the present study, the effect of cavitation (growth and violent collapse of vapour bubbles in a liquid) on delignification of wood was investigated. Steam was introduce...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.01.042

    authors： Baxi PB,Pandit AB

    更新日期：2012-04-01 00:00:00