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Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae.

Abstract：

:In this study, five recombinant Saccharomyces cerevisiae strains were compared for their xylose-fermenting ability. The most efficient xylose-to-ethanol fermentation was found by using the industrial strain MA-R4, in which the genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis along with an endogenous xylulokinase gene were expressed by chromosomal integration of the flocculent yeast strain IR-2. The MA-R4 strain rapidly converted xylose to ethanol with a low xylitol yield. Furthermore, the MA-R4 strain had the highest ethanol production when fermenting not only a mixture of glucose and xylose, but also mixed sugars in the detoxified hydrolysate of wood chips. These results collectively suggest that MA-R4 may be a suitable recombinant strain for further study into large-scale ethanol production from mixed sugars present in lignocellulosic hydrolysates.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Matsushika A,Inoue H,Murakami K,Takimura O,Sawayama S

doi

10.1016/j.biortech.2008.11.047

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

2392-8

issue

8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(08)01025-0

journal_volume

100

pub_type

杂志文章

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