Abstract:
:In this study, a newly strain named Clostridium butyricum YJH-09 were isolated from the sample of pond soil and identified through physiological, biochemical and 16S rDNA analysis. Then, the dhaT gene encoding a novel 1,3-propanediol dehydrogenase (PDOR) was cloned from this strain and expressed in Escherichia coli BL21(DE3). Subsequently, the recombinant PDOR was purified and the optimal pH and temperature, specific activities and kinetic parameter were investigated. Furthermore, the whole cells of Clostridium butyricum YJH-09 mixed with BL21-dhaT were used to produce 1,3-PD through co-biotransformation. As results, 25.88g/L of 1,3-PD was generated with 0.54g/g yield from 50g/L glycerol in 30h, and the 1,3-PD production was increased more than 2-fold compared with wild type strain alone. This research would offer useful information for further development of the biosynthesis of 1,3-PD.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Yun J,Yang M,Magocha TA,Zhang H,Xue Y,Zhang G,Qi X,Sun Wdoi
10.1016/j.biortech.2017.09.180subject
Has Abstractpub_date
2018-01-01 00:00:00pages
838-843eissn
0960-8524issn
1873-2976pii
S0960-8524(17)31756-Xjournal_volume
247pub_type
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