Improved production of heterologous lipase in Trichoderma reesei by RNAi mediated gene silencing of an endogenic highly expressed gene.

Abstract:

:A lipase gene (Lip) of the Aspergillus niger was de novo synthesized and expressed in the Trichoderma reesei under the promoter of the cellobiohydrolase I gene (cbh1). RNAi-mediated gene silencing was successfully used to further improve the recombinant lipase production via down-regulation of CBHI which comprised more than 60% of the total extracellular proteins in T. reesei. The gene and protein expression of CBHI and recombinant lipase were analyzed by real-time PCR, SDS-PAGE and activity assay. The results demonstrated that RNAi-mediated gene silencing could effectively suppress cbh1 gene expression and the reduction of CBHI could result in obvious improvement of heterologous lipase production. The reconstructed strains with decreased CBHI production exhibited 1.8- to 3.2-fold increase in lipase activity than that of parental strain. The study herein provided a feasible and advantageous method of increasing heterologous target gene expression in T. reesei through preventing the high expression of a specific endogenenous gene by RNA interference.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Qin LN,Cai FR,Dong XR,Huang ZB,Tao Y,Huang JZ,Dong ZY

doi

10.1016/j.biortech.2012.01.013

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

116-22

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)00019-3

journal_volume

109

pub_type

杂志文章
  • Biological removal of nitrogen oxides by microalgae, a promising strategy from nitrogen oxides to protein production.

    abstract::Nitrogen oxides (NOx) are the components of fossil flue gases that give rise to serious environmental and health hazards. Among the available techniques for NOx removal, microalgae-based biological removal of NOx (BioDeNOx) is a promising and competent technology with eco-friendly path of low energy and low-cost solut...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2019.122037

    authors: Qie F,Zhu J,Rong J,Zong B

    更新日期:2019-11-01 00:00:00

  • Effect of organic carbon and nutrient supplementation on the digestate-grown microalga, Parachlorella kessleri.

    abstract::Digested effluents are usually deprived of the appropriate levels of organic carbon or macro- and micro-nutrients to effectively sustain microalgal growth. In this regard, Parachlorella kessleri was cultivated in an agro-waste digestate supplemented with different glucose concentrations, magnesium and trace metals and...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.122232

    authors: Koutra E,Kopsahelis A,Maltezou M,Grammatikopoulos G,Kornaros M

    更新日期:2019-12-01 00:00:00

  • Enhancing growth and lipid production of marine microalgae for biodiesel production via the use of different LED wavelengths.

    abstract::Wavelength of light is a crucial factor which renders microalgae as the potential biodiesel. In this study, Tetraselmis sp. and Nannochloropsis sp. as famous targets were selected. The effect of different light wavelengths on growth rate and lipid production was studied. Microalgae were cultivated for 14 days as under...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.03.113

    authors: Teo CL,Atta M,Bukhari A,Taisir M,Yusuf AM,Idris A

    更新日期:2014-06-01 00:00:00

  • Kinetics of phosphorus and potassium release from rock phosphate and waste mica enriched compost and their effect on yield and nutrient uptake by wheat (Triticum aestivum).

    abstract::An attempt was made to study the efficient use of rice straw and indigenous source of phosphorus and potassium in crop production through composting technology. Various enriched composts were prepared using rice straw, rock phosphate (RP), waste mica and bioinoculant (Aspergillus awamori) and kinetics of release of ph...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.08.025

    authors: Nishanth D,Biswas DR

    更新日期:2008-06-01 00:00:00

  • Identification and quantification of glycoside flavonoids in the energy crop Albizia julibrissin.

    abstract::Oxygen radical absorbance capacity (ORAC) values showed that methanolic extracts of Albizia julibrissin foliage displayed antioxidant activity. High performance liquid chromatography (HPLC) and mass spectrometry (MS) techniques were utilized in the identification of the compounds. The analysis confirmed the presence o...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2005.12.011

    authors: Lau CS,Carrier DJ,Beitle RR,Bransby DI,Howard LR,Lay JO Jr,Liyanage R,Clausen EC

    更新日期:2007-01-01 00:00:00

  • Improvement of gaseous energy recovery from sugarcane bagasse by dark fermentation followed by biomethanation process.

    abstract::The aim of the present study was to enhance the gaseous energy recovery from sugarcane bagasse. The two stage (biohydrogen and biomethanation) batch process was considered under mesophilic condition. Alkali pretreatment (ALP) was used to remove lignin from sugarcane bagasse. This enhanced the enzymatic digestibility o...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.07.038

    authors: Kumari S,Das D

    更新日期:2015-10-01 00:00:00

  • Removal of metal and organic pollutants from wastewater by a sequential selective technique.

    abstract::In this study the application of a sequential selective system that combined biosorption with biodegradation was evaluated as a feasible process for the removal of Cr(VI) and m-cresol from effluents. Cr(VI) biosorption on pretreated chestnut shells showed 100% metal removal and modelling efforts demonstrated that the ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.02.036

    authors: Cobas M,Danko AS,Pazos M,Sanromán MA

    更新日期:2016-08-01 00:00:00

  • Impact of copper on the diazotroph abundance and community composition during swine manure composting.

    abstract::Biological nitrogen fixation is a major pathway in ecosystems. This study investigated the effects of adding Cu at different levels (0, 200, and 2000 mg kg-1) on the diazotroph community during swine manure composting. Quantitative PCR and high-throughput sequencing were used to analyze the abundances of diazotrophs a...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.01.120

    authors: Yin Y,Gu J,Wang X,Zhang K,Hu T,Ma J,Wang Q

    更新日期:2018-05-01 00:00:00

  • Recent developments on algal biochar production and characterization.

    abstract::Algal biomass is known as a promising sustainable feedstock for the production of biofuels and other valuable products. However, since last decade, massive amount of interests have turned to converting algal biomass into biochar. Due to their high nutrient content and ion-exchange capacity, algal biochars can be used ...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2017.08.009

    authors: Yu KL,Lau BF,Show PL,Ong HC,Ling TC,Chen WH,Ng EP,Chang JS

    更新日期:2017-12-01 00:00:00

  • Photosynthetic light reactions increase total lipid accumulation in carbon-supplemented batch cultures of Chlorella vulgaris.

    abstract::Microalgae are an attractive biofuel feedstock because of their high lipid to biomass ratios, lipid compositions that are suitable for biodiesel production, and the ability to grow on varied carbon sources. While algae can grow autotrophically, supplying an exogenous carbon source can increase growth rates and allow h...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.11.098

    authors: Woodworth BD,Mead RL,Nichols CN,Kolling DRJ

    更新日期:2015-03-01 00:00:00

  • Water soluble fractions of rose-scented geranium (Pelargonium species) essential oil.

    abstract::The essential oil of rose-scented geranium (Pelargonium species, family: Geraniaceae) obtained through steam or water plus steam distillation of shoot biomass is extensively used in the fragrance industry and in aromatherapy. During distillation, a part of the essential oil becomes dissolved in the distillation water ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/s0960-8524(02)00057-3

    authors: Rao BR,Kaul PN,Syamasundar KV,Ramesh S

    更新日期:2002-09-01 00:00:00

  • Metabolic pathways for microalgal biohydrogen production: Current progress and future prospectives.

    abstract::Microalgal biohydrogen (bioH2) has attracted global interest owing to its potential carbon-free source of sustainable renewable energy. Most of previous reviews which focused on microalgal bioH2, have shown unclear differentiation among the metabolic pathways. In this review, investigation of all different metabolic p...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2020.124253

    authors: El-Dalatony MM,Zheng Y,Ji MK,Li X,Salama ES

    更新日期:2020-12-01 00:00:00

  • Characterization of five agricultural by-products as potential biofilter carriers.

    abstract::Biofiltration is the most commonly used biological gas treatment technology and is extensively used for the treatment of polluted air with gas flow rates of up to 2 x 10(5) m(3)/h. It involves a filter bed of organic matter serving both as carrier for microorganisms and as nutrient supplier. Polluted gas passes throug...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/s0960-8524(02)00315-2

    authors: Ramírez-López E,Corona-Hernández J,Dendooven L,Rangel P,Thalasso F

    更新日期:2003-07-01 00:00:00

  • Study of moving bed biofilm reactor in diethyl phthalate and diallyl phthalate removal from synthetic wastewater.

    abstract::Phthalic acid esters have received significant attention over the last few years since they are considered as priority pollutants. In this study, effects of different operation conditions including hydraulic retention time, phthalates loading rates and aeration rate on process performance of moving bed biofilm reactor...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.01.122

    authors: Ahmadi E,Gholami M,Farzadkia M,Nabizadeh R,Azari A

    更新日期:2015-05-01 00:00:00

  • Improving abiotic reducing ability of hydrothermal biochar by low temperature oxidation under air.

    abstract::Oxidized hydrothermal biochar was prepared by hydrothermal carbonization of Spartina alterniflora biomass (240°C for 4h) and subsequent oxidization (240°C for 10min) under air. Oxidized hydrochar achieved a Fe(III) reducing capacity of 2.15mmol/g at pH 2.0 with 120h, which is 1.2 times higher than un-oxidized hydrocha...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.09.018

    authors: Xu Y,Lou Z,Yi P,Chen J,Ma X,Wang Y,Li M,Chen W,Liu Q,Zhou J,Zhang J,Qian G

    更新日期:2014-11-01 00:00:00

  • Converting paper mill sludge into neutral lipids by oleaginous yeast Cryptococcus vishniaccii for biodiesel production.

    abstract::Paper mill sludge (PMS) was assessed as cheap renewable lignocellulosic biomass for lipid production by the oleaginous yeast Cryptococcus vishniaccii (MTCC 232). The sonicated paper mill sludge extract (PMSE) exhibited enhanced lipid yield and lipid content 7.8±0.57g/l, 53.40% in comparison to 5.5±0.8g/l, 40.44% gluco...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.02.105

    authors: Deeba F,Pruthi V,Negi YS

    更新日期:2016-08-01 00:00:00

  • Cultivation of Chlorella sp. using raw dairy wastewater for nutrient removal and biodiesel production: Characteristics comparison of indoor bench-scale and outdoor pilot-scale cultures.

    abstract::The biomass productivity and nutrient removal capacity of simultaneous Chlorella sp. cultivation for biodiesel production and nutrient removal in raw dairy wastewater (RDW) in indoor bench-scale and outdoor pilot-scale photobioreactors were compared. Results from the current work show that maximum biomass productivity...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.05.094

    authors: Lu W,Wang Z,Wang X,Yuan Z

    更新日期:2015-09-01 00:00:00

  • Removal of pharmaceuticals in conventionally treated wastewater by a polishing moving bed biofilm reactor (MBBR) with intermittent feeding.

    abstract::Previous studies have demonstrated that aerobic moving bed biofilm reactors (MBBRs) remove pharmaceuticals better than activated sludge. Thus we used a MBBR system to polish the effluent of an activated sludge wastewater treatment plant. To overcome that effluent contains insufficient organic matter to sustain enough ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.03.159

    authors: Tang K,Ooi GTH,Litty K,Sundmark K,Kaarsholm KMS,Sund C,Kragelund C,Christensson M,Bester K,Andersen HR

    更新日期:2017-07-01 00:00:00

  • Management of food industry waste employing vermicomposting technology.

    abstract::This paper reports the vermicomposting of food industry sludges (FIS) mixed with different organic wastes employing Eisenia fetida. A total of 10 vermicomposting units containing different wastes combinations were established. After 15 weeks significant increase in total nitrogen (N(total)) (60-214%), total available ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2011.11.116

    authors: Garg VK,Suthar S,Yadav A

    更新日期:2012-12-01 00:00:00

  • Acclimation of Nannochloropsis gaditana to different illumination regimes: effects on lipids accumulation.

    abstract::Algae are interesting potential sources of biodiesel, although research is still needed to develop efficient large scale productions. One major factor affecting productivity is light use efficiency. The effect of different light regimes on the seawater alga Nannochloropsis gaditana was accessed monitoring growth rate ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2011.02.100

    authors: Simionato D,Sforza E,Corteggiani Carpinelli E,Bertucco A,Giacometti GM,Morosinotto T

    更新日期:2011-05-01 00:00:00

  • Comparative study of the efficiency of synthetic and natural mediators in laccase-assisted bleaching of eucalyptus kraft pulp.

    abstract::The natural phenolic compounds syringaldehyde and vanillin were compared to the synthetic mediators 1-hydroxybenzotriazole, violuric acid and promazine in terms of boosting efficiency in a laccase-assisted biobleaching of eucalyptus kraft pulp. Violuric acid and 1-hydroxybenzotriazole revealed to be the most effective...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2008.04.002

    authors: Moldes D,Díaz M,Tzanov T,Vidal T

    更新日期:2008-11-01 00:00:00

  • Influence of various nitrogenous electron acceptors on the anaerobic sulfide oxidation.

    abstract::The effect of nitrate and nitrite as electron acceptors on the performance of anaerobic sulfide oxidizing process (ASO process) was investigated. For nitrate-ASO process, the maximum influent nitrogen concentration was lower than that in nitrite-ASO process, but the maximum influent sulfur concentration was higher tha...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.11.047

    authors: Jing C,Ping Z,Mahmood Q

    更新日期:2010-05-01 00:00:00

  • Bio-hythane production from microalgae biomass: Key challenges and potential opportunities for algal bio-refineries.

    abstract::The interest in microalgae for wastewater treatment and liquid bio-fuels production (i.e. biodiesel and bioethanol) is steadily increasing due to the energy demand of the ultra-modern technological world. The associated biomass and by-product residues generated from these processes can be utilized as a feedstock in an...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2017.05.156

    authors: Ghimire A,Kumar G,Sivagurunathan P,Shobana S,Saratale GD,Kim HW,Luongo V,Esposito G,Munoz R

    更新日期:2017-10-01 00:00:00

  • Biocatalytic production of (2S,3S)-2,3-butanediol from diacetyl using whole cells of engineered Escherichia coli.

    abstract::(2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a crucial chiral compound that acts as an excellent building block in asymmetric synthesis of highly valuable chiral compounds. However, the low concentration and optical purity of (2S,3S)-2,3-BD produced in previous studies limited its applications. In the present work, the ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2011.08.097

    authors: Li L,Wang Y,Zhang L,Ma C,Wang A,Tao F,Xu P

    更新日期:2012-07-01 00:00:00

  • Comparative study of lipid extraction from microalgae by organic solvent and supercritical CO2.

    abstract::Pavlova sp. was employed to evaluate the efficiency of different lipid extraction methods. The microalgal crude lipids content determined using the mixed solvent with ultrasonic method was 44.7 wt.%. The triglyceride content obtained by the mixed solvent method was 15.6 wt.%. The extraction yield was the FAME yield di...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2011.08.064

    authors: Cheng CH,Du TB,Pi HC,Jang SM,Lin YH,Lee HT

    更新日期:2011-11-01 00:00:00

  • Kinetics evaluation and thermal decomposition characteristics of co-pyrolysis of municipal sewage sludge and hazelnut shell.

    abstract::Hazelnut shell, as novel biomass, has lower ash content and abundant hydrocarbon, which can be utilized resourcefully with municipal sewage sludge (MSS) by co-pyrolyisis to decrease total content of pollution. The co-pyrolysis of MSS and hazelnut shell blend was analyzed by a method of multi-heating rates and differen...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.09.008

    authors: Zhao B,Xu X,Li H,Chen X,Zeng F

    更新日期:2018-01-01 00:00:00

  • Quantification of nitrous oxide (N2O) emissions and soluble microbial product (SMP) production by a modified AOB-NOB-N2O-SMP model.

    abstract::A modified AOB-NOB-N2O-SMP model able to quantify nitrous oxide (N2O) emissions and soluble microbial product (SMP) production during wastewater treatment is proposed. The modified AOB-NOB-N2O-SMP model takes into account: (1) two-step nitrification by ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (N...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.11.127

    authors: Kim M,Wu G,Yoo C

    更新日期:2017-03-01 00:00:00

  • Innovative nanocomposite formulations for enhancing biogas and biofertilizers production from anaerobic digestion of organic waste.

    abstract::Herein, the design of nanocomposite (NC) formulations that consist of metal enzyme cofactors, highly conductive carbon materials, DIET activators, to boost AD biogas production from anaerobically incubated cattle manure are investigated and discussed. Three different NC formulations were designed and synthesized: zinc...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2020.123350

    authors: Hassaneen FY,Abdallah MS,Ahmed N,Taha MM,Abd ElAziz SMM,El-Mokhtar MA,Badary MS,Allam NK

    更新日期:2020-08-01 00:00:00

  • Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment.

    abstract::To overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature wi...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.122568

    authors: Huang C,Fang G,Yu L,Zhou Y,Meng X,Deng Y,Shen K,Ragauskas AJ

    更新日期:2020-03-01 00:00:00

  • Plausible exploitation of Jatropha de-oiled seed cake for lipase and phytase production and simultaneous detoxification by Candida parapsilosis isolated from poultry garbage.

    abstract::Jatropha de-oiled seed cake was explored to utilize as a basic nutrient source for Candida parapsilosis, isolated from poultry garbage and selected based on the production of lipase and phytase enzymes under submerged fermentation. At optimized parameters under solid-state fermentation, lipase and phytase activities w...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.11.065

    authors: Kannoju B,Ganapathiwar S,Nunavath H,Sunkar B,Bhukya B

    更新日期:2017-02-01 00:00:00