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Optimization of fed-batch enzymatic hydrolysis from alkali-pretreated sugarcane bagasse for high-concentration sugar production.

Abstract：

:Fed-batch enzymatic hydrolysis process from alkali-pretreated sugarcane bagasse was investigated to increase solids loading, produce high-concentration fermentable sugar and finally to reduce the cost of the production process. The optimal initial solids loading, feeding time and quantities were examined. The hydrolysis system was initiated with 12% (w/v) solids loading in flasks, where 7% fresh solids were fed consecutively at 6h, 12h, 24h to get a final solids loading of 33%. All the requested cellulase loading (10 FPU/g substrate) was added completely at the beginning of hydrolysis reaction. After 120 h of hydrolysis, the maximal concentrations of cellobiose, glucose and xylose obtained were 9.376 g/L, 129.50 g/L, 56.03 g/L, respectively. The final total glucan conversion rate attained to 60% from this fed-batch process.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Gao Y,Xu J,Yuan Z,Zhang Y,Liu Y,Liang C

doi

10.1016/j.biortech.2014.05.034

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

41-5

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)00693-2

journal_volume

167

pub_type

杂志文章

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