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Statistical optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16.

Abstract：

:The optimization of culture condition for enhanced hydrogen production by Thermoanaerobacterium thermosaccharolyticum W16 was conducted using statistical experimental design and analysis. Plackett-Burman design was first used to screen the most important variables influencing hydrogen production, and subsequently central composite design was adopted to investigate the optimum value of the selected factors for achieving maximum hydrogen yield. Experimental results showed that xylose, phosphate buffer, and yeast extract had significant influence on hydrogen production and the maximum hydrogen yield of 2.39 mol/mol xylose was predicted when the concentrations of xylose, phosphate buffer, and yeast extract were 12.24 g/L, 0.170 M, and 4.11 g/L, respectively. The results were further verified by repeated experiments under optimal conditions. The excellent correlation between predicted and measured values further confirmed the validity and practicability of this statistical optimum strategy.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Cao GL,Ren NQ,Wang AJ,Guo WQ,Yao J,Feng YJ,Zhao QL

doi

10.1016/j.biortech.2009.11.031

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

2053-8

issue

6

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(09)01535-1

journal_volume

101

pub_type

杂志文章

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