Abstract:
:In this study, the factors affecting ferulic acid (FA) release from Brewer's spent grain (BSG), by the crude enzyme extract of Fusarium oxysporum were investigated. In order to evaluate the importance of the multienzyme preparation on FA release, the synergistic action of feruloyl esterase (FAE, FoFaeC-12213) and xylanase (Trichoderma longibrachiatum M3) monoenzymes was studied. More than double amount of FA release (1 mg g(-1) dry BSG) was observed during hydrolytic reactions by the crude enzyme extract compared to hydrolysis by the monoenzymes (0.37 mg g(-1) dry BSG). The protease content of the crude extract and the inhibitory effect of FA as an end-product were also evaluated concerning their effect on FA release. The protease treatment prior to hydrolysis by monoenzymes enhanced FA release about 100%, while, for the first time in literature, FA in solution found to have a significant inhibitory effect on FAE activity and on total FA release.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Xiros C,Moukouli M,Topakas E,Christakopoulos Pdoi
10.1016/j.biortech.2009.06.018subject
Has Abstractpub_date
2009-12-01 00:00:00pages
5917-21issue
23eissn
0960-8524issn
1873-2976pii
S0960-8524(09)00675-0journal_volume
100pub_type
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