Abstract：
:The protective effect and the mechanisms of trehalose accumulation in industrial Saccharomyces cerevisiae strains were investigated during ethanol fermentation. The engineered strains with more intercellular trehalose achieved significantly higher fermentation rates and ethanol yields than their wild strain ZS during very high gravity (VHG) fermentation, while their performances were not different during regular fermentation. The VHG fermentation performances of these strains were consistent with their growth capacity under osmotic stress and ethanol stress, the key stress factors during VHG fermentation. These results suggest that trehalose accumulation is more important for VHG fermentation of industrial yeast strains than regular one. The differences in membrane integrity and antioxidative capacity of these strains indicated the possible mechanisms of trehalose as a protectant under VHG condition. Therefore, trehalose metabolic engineering may be a useful strategy for improving the VHG fermentation performance of industrial yeast strains.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Wang PM,Zheng DQ,Chi XQ,Li O,Qian CD,Liu TZ,Zhang XY,Du FG,Sun PY,Qu AM,Wu XCdoi
10.1016/j.biortech.2013.11.033subject
Has Abstractpub_date
2014-01-01 00:00:00pages
371-6eissn
0960-8524issn
1873-2976pii
S0960-8524(13)01749-5journal_volume
152pub_type
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