Abstract:
:The effect of temperature on the functionality of microbial community structure in a low temperature, anaerobic fixed-bed reactor was studied by decreasing the operating temperature from 18 °C to 5 °C. The reactor was productive within 20 days and produced stable methane content in biogas (above 77%) throughout the trial period. At 17 °C and 15 °C, chemical oxygen demand (COD) removal efficiency and biogas production of reactor were significantly reduced. These might be temperature thresholds when fixed-bed reactors are operated under low temperatures. The methanogen community composition was analyzed using 16S rRNA gene clone library screening and quantitative PCR. At low ambient temperatures, Methanomicrobiales were dominant methanogens, and they preferentially adhered to the carbon fiber carrier. The results indicated that 16S rRNA levels of Methanomicrobiales and Methanosaetaceae in adhering sludge were higher than in deposited sludge, and they all contributed to the efficient performance of the fixed-bed reactor at low operating temperatures.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhang D,Zhu W,Tang C,Suo Y,Gao L,Yuan X,Wang X,Cui Zdoi
10.1016/j.biortech.2011.10.086subject
Has Abstractpub_date
2012-01-01 00:00:00pages
136-43eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01574-4journal_volume
104pub_type
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