Abstract：
:This study has assessed four invasive weeds, viz. Saccharum spontaneum (SS), Mikania micrantha (MM), Lantana camara (LC) and Eichhornia crassipes (EC) for enzymatic hydrolysis prior to bioalcohol fermentation. Enzymatic hydrolysis of pretreated biomasses of weeds has been conducted with mechanical agitation and sonication under constant (non-optimum) conditions. Profiles of total reducible sugar release have been fitted to HCH-1 model of enzymatic hydrolysis using Genetic Algorithm. Trends in parameters of this model reveal physical mechanism of ultrasound-induced enhancement of enzymatic hydrolysis. Sonication accelerates hydrolysis kinetics by ∼10-fold. This effect is contributed by several causes, attributed to intense micro-convection generated during sonication: (1) increase in reaction velocity, (2) increase in enzyme-substrate affinity, (3) reduction in product inhibition, and (4) enhancement of enzyme activity due to conformational changes in its secondary structure. Enhancement effect of sonication is revealed to be independent of conditions of enzymatic hydrolysis - whether optimum or non-optimum.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Borah AJ,Agarwal M,Poudyal M,Goyal A,Moholkar VSdoi
10.1016/j.biortech.2016.02.024subject
Has Abstractpub_date
2016-08-01 00:00:00pages
342-349eissn
0960-8524issn
1873-2976pii
S0960-8524(16)30150-Xjournal_volume
213pub_type
杂志文章abstract：:The soil bacterium Arthrobacter aurescens SK 8.001 produces inulin fructotransferase (IFTase), and liquid chromatography-mass spectrometry (LC-MS) and carbon-13 nuclear magnetic resonance (13C NMR) analysis demonstrated that the main product of the enzyme was difructose anhydride III (DFA III). The IFTase was purified...
journal_title：Bioresource technology
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doi：10.1016/j.biortech.2014.02.039
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更新日期：2011-01-01 00:00:00
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更新日期：2011-04-01 00:00:00
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更新日期：2015-01-01 00:00:00
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更新日期：2016-04-01 00:00:00
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更新日期：2017-10-01 00:00:00
