Abstract：
:Douglas-fir was SO(2)-steam pretreated at different severities (190, 200, and 210°C) to assess the possible negative effect of the residual and isolated lignins on the enzymatic hydrolysis of the steam pretreated substrates. When various isolated lignins were added to the Avicel hydrolysis reactions, the decrease in glucose yields ranged from 15.2% to 29.0% after 72 h. It was apparent that the better hydrolysis yields obtained at higher pretreatment severities were more a result of the greater accessibly of the cellulose rather than any specific change in the non-productive binding of the lignin to the enzymes. FTIR and (13)C NMR characterization indicated that the lignin in the steam pretreated substrates became more condensed with increasing severity, suggesting that the cellulases were adsorbed to the lignin by hydrophobic interactions. Electrostatic interactions were also involved as the positively charged cellulase components were preferentially adsorbed to the lignins.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Nakagame S,Chandra RP,Kadla JF,Saddler JNdoi
10.1016/j.biortech.2010.12.082subject
Has Abstractpub_date
2011-03-01 00:00:00pages
4507-17issue
6eissn
0960-8524issn
1873-2976pii
S0960-8524(10)02067-5journal_volume
102pub_type
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