Abstract：
:The soil bacterium Arthrobacter aurescens SK 8.001 produces inulin fructotransferase (IFTase), and liquid chromatography-mass spectrometry (LC-MS) and carbon-13 nuclear magnetic resonance (13C NMR) analysis demonstrated that the main product of the enzyme was difructose anhydride III (DFA III). The IFTase was purified by ethanol precipitation, DEAE Sepharose Fast Flow, and Superdex 200 10/300 GL gel chromatography. Its molecular mass was estimated to be 40 kDa by SDS-PAGE and 35 kDa by gel filtration. The enzyme showed maximum activity at pH 5.5 and 60-70 °C, and retained 86.5% of its initial activity after incubation at 60 °C for 4 h. Chemical modification results suggested that a tryptophan residue is essential to enzyme activity. The N-terminal amino acid sequence was determined as AEGAKASPLNSPNVYDVT. The kinetic values, Km and Vmax, were estimated to be 0.52 mM and 0.3 μmol/ml min. Nystose was observed to be the smallest substrate for the produced IFTase. This IFTase provides a promising way to utilize inulin for the production of DFA III.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhao M,Mu W,Jiang B,Zhou L,Zhang T,Lu Z,Jin Z,Yang Rdoi
10.1016/j.biortech.2010.08.093subject
Has Abstractpub_date
2011-01-01 00:00:00pages
1757-64issue
2eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01478-1journal_volume
102pub_type
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