Abstract：
:A mutant strain of Klebsiella pneumoniae, termed GEM167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. Levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-PD) and 3-hydroxypropionic acid (3-HP), were decreased in the GEM167 strain compared to a control strain, whereas the levels of metabolites derived from the oxidative pathway, 2,3-butanediol (2,3-BD), ethanol, lactate, and succinate, were increased. Notably, ethanol production from glycerol was greatly enhanced upon fermentation by the mutant strain, to a maximum production level of 21.5 g/l, with a productivity of 0.93 g/l/h. Ethanol production level was further improved to 25.0 g/l upon overexpression of Zymomonas mobilis pdc and adhII genes encoding pyruvate decarboxylase (Pdc) and aldehyde dehydrogenase (Adh), respectively in the mutant strain GEM167.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Oh BR,Seo JW,Heo SY,Hong WK,Luo LH,Joe MH,Park DH,Kim CHdoi
10.1016/j.biortech.2010.12.007subject
Has Abstractpub_date
2011-02-01 00:00:00pages
3918-22issue
4eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01937-1journal_volume
102pub_type
杂志文章abstract：:The present work examines the production of a biodiesel from a non-edible oil namely honne oil (Calophyllum inophyllum linn). A three stage process viz., pre-treatment, alkali catalyzed transesterification and post treatment adopted for the production is discussed. The reaction parameters such as methanol to oil molar...
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