Abstract：
:A strain, Stenotrophomonas HPC383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through HPLC and GC analysis. Culture HPC383 could degrade (%) various compounds (1 mM) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% crude oil. Gene locus dmpN, to identify phenol degrading capacity was determined by PCR followed by southern analysis. The sequenced DNA fragment exhibited 99% sequence similarity to phenol hydroxylase gene from Arthrobacter sp. W1 (FJ610336). Amino acid sequence analysis of phenol hydroxylase reveals it to belong to high-Ks (affinity constant) group. Application of HPC383 in bioremediation of aquatic and terrestrial sites contaminated with petrochemical has been suggested.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Verma V,Raju SC,Kapley A,Kalia VC,Kanade GS,Daginawala HF,Purohit HJdoi
10.1016/j.biortech.2010.11.016subject
Has Abstractpub_date
2011-02-01 00:00:00pages
3227-33issue
3eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01818-3journal_volume
102pub_type
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