Abstract：
:Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45FPU/g cellulose.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Guo H,Zou S,Liu B,Su R,Huang R,Qi W,Zhang M,He Zdoi
10.1016/j.biortech.2015.03.105subject
Has Abstractpub_date
2015-01-01 00:00:00pages
362-368eissn
0960-8524issn
1873-2976pii
S0960-8524(15)00437-Xjournal_volume
187pub_type
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