Chemical and enzymatic sequential pretreatment of oat straw for methane production.

Abstract:

:Oat straw was subjected to sequential pretreatment: acid/alkaline/enzymatic, to convert the lignocellulosic material in soluble sugars. The hydrolysates from acid pretreatment (2% HCl, 90 °C) and enzymatic pretreatment (cellulase, pH 4.5, 45 °C) were used as substrates in two lab-scale UASB reactors for methane production. The acid and enzymatic hydrolysates contained 25.6 and 35.3g/L of total sugars, respectively, which corresponded to a COD of 23.6 and 30.5 g/L, respectively. The UASB reactor fed with acid hydrolysate achieved a maximum methane yield of 0.34 L CH(4)/g COD at an organic loading rate (OLR) of 2.5 g COD/L-d. In the reactor fed with enzymatic hydrolysate the methane yield was 0.36 LCH(4)/g COD at OLR higher than 8.8 g COD/L-d. The anaerobic digestion of both hydrolysates was feasible without the need of a detoxification step. The sequential pretreatment of oat straw allowed to solubilize 96.8% of hemicellulose, 77.2% of cellulose and 42.2% of lignin.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Gomez-Tovar F,Celis LB,Razo-Flores E,Alatriste-Mondragón F

doi

10.1016/j.biortech.2012.03.109

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

372-8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)00573-1

journal_volume

116

pub_type

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