Investigations into the biodegradation of microcystin-LR mediated by the biofilm in wintertime from a biological treatment facility in a drinking-water treatment plant.

Abstract:

:The potential of winter biofilm for microcystin-LR (MCLR) biodegradation was comparatively evaluated under various nutrient conditions. Results indicated that MCLR was completely biodegraded by Day 7 without nutrient addition. MCLR-biodegradation was inhibited in the presence of phosphate or glucose addition, with complete MCLR removal observed by Day 10. MCLR was totally biodegraded by Day 7 with dual nutrients comprising glucose and nitrate, suggesting that additional nitrate alleviated the inhibitory effect of glucose alone on the biodegradation. Simultaneously, MCLR-degrading gene (mlrA) abundance were detected to increase with increasing amount of MCLR being degraded under the respective conditions, implying that MCLR-biodegradation depended on the population of indigenous MCLR-degrading bacteria (MCLRDB), which was related to the population of non-degrading bacteria in the biofilm. MCLRDB was found to primarily use MCLR for proliferation rather than other nutrients. This is the first report verifying MCLR as a primary substrate for bacteria under various nutrient conditions.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Li J,Shimizu K,Maseda H,Lu Z,Utsumi M,Zhang Z,Sugiura N

doi

10.1016/j.biortech.2011.11.099

subject

Has Abstract

pub_date

2012-02-01 00:00:00

pages

27-35

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)01710-X

journal_volume

106

pub_type

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