Bioethanol production from Lantana camara (red sage): Pretreatment, saccharification and fermentation.

Abstract:

:Lantanacamara contains 61.1% (w/w) holocellulose and can serve as a low-cost feedstock for bioethanol production. Acid hydrolysis (3.0%, v/v H(2)SO(4), 120 degrees C for 45 min) of L. camara produced 187.14 mg/g total sugars along with fermentation inhibitors such as phenolics (8.2mg/g), furfurals (5.1mg/g) and hydroxy methyl furfurals (6.7 mg/g). Sequential application of overliming (pH 10.0) and activated charcoal (1.5%, w/v) adsorption was used to remove these toxic compounds from the acid hydrolysate. The acid-pretreated biomass of L. camara was further delignified through combined pretreatment of sodium sulphite (5.0% w/v) and sodium chlorite (3.0% w/v), which resulted in about 87.2% lignin removal. The enzymatic hydrolysis of delignified cellulosic substrate showed 80.0% saccharification after 28 h incubation at 50 degrees C and pH 5.0. Fermentation of acid and enzymatic hydrolysates with Pichiastipitis and Saccharomycescerevisiae gave rise to 5.16 and 17.7 g/L of ethanol with corresponding yields of 0.32 and 0.48 g/g after 24 and 16 h, respectively.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Kuhad RC,Gupta R,Khasa YP,Singh A

doi

10.1016/j.biortech.2010.06.043

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

8348-54

issue

21

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)01024-2

journal_volume

101

pub_type

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