Effect of operating temperatures on the microbial community profiles in a high cell density hybrid anaerobic bioreactor.

Abstract:

:Lack of knowledge about the microbial consortia involved in wastewater treatment at different operating temperatures, is a major reason for failure of anaerobic reactors in field applications. Present study was undertaken to correlate performance of hybrid anaerobic reactors operating at different temperatures (37, 45 and 55 °C) to structures of archaeal and bacterial communities involved. Self-immobilized granules were developed in the reactors continuously fed with synthetic wastewater (10,000 mg COD l(-1)) and operated at an organic loading rate of 2.22 kg COD m(-3) day(-1) and hydraulic retention time of 5 days. The reactor operated at 37 °C showed the best performance as well as the most diverse microbial community revealed by PCR-denaturing gradient gel electrophoresis analysis using 16S rRNA gene amplicons. Sequences derived from reactors operating at higher temperatures revealed presence of different methanogens, but lesser diversity caused a drop in COD degradation capability of the system indicating successful operation at low loading conditions.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Kundu K,Sharma S,Sreekrishnan TR

doi

10.1016/j.biortech.2012.05.047

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

502-11

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)00791-2

journal_volume

118

pub_type

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