Biotransformation of ferulic acid to 4-vinylguaiacol by a wild and a diploid strain of Aspergillus niger.

Abstract:

:Ferulic acid biotransformation has a number of interesting industrial uses. Ferulic acid biotransformation by the wild strain Aspergillus niger C28B25 and a diploid strain DAR2, obtained by parasexual recombination, was studied. The wild strain of A.niger C28B25 biotransforms ferulic acid to vanillic acid (VA); while the diploid strain DAR2 preferentially decarboxylates ferulic acid to 4-vinylguaiacol (4VG). The latter was identified by mass spectroscopy, (1)H and (13)C nuclear magnetic resonance spectroscopy, and quantified by HPLC. The diploid strain A.niger DAR2 and the wild strain showed a ferulic acid conversion of 64% and 36%, respectively. Molar yields show that the formation of 4VG was preferred, being as much as 4.4 times higher than the formation of VA in diploid strain cultures. Differential regulation of enzymes involved in the biotransformation of ferulic acid may explain the accumulation of 4VG by diploid DAR2. This strain produced both 4VG and VA.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Baqueiro-Peña I,Rodríguez-Serrano G,González-Zamora E,Augur C,Loera O,Saucedo-Castañeda G

doi

10.1016/j.biortech.2010.01.086

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

4721-4

issue

12

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)00172-0

journal_volume

101

pub_type

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