Abstract：
:Extents of adsorption of cellulolytic enzymes on lignin, derived from sugarcane bagasse, were an inverse function of incubation temperature and varied with type of lignin extraction. At 45 °C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbed cellulolytic enzymes from Trichoderma reesei within 90 min. Lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbed only 60% of T. reesei endoglucanase, exoglucanase and β-glucosidase activities. β-Glucosidase from Aspergillus niger was not adsorbed. At 30 °C, adsorption of all of the enzymes was minimal and enzyme hydrolysis at 30 °C approached that at 45 °C after 168 h. Hence, temperature provided an approach to decrease loss of enzyme activity by reducing enzyme adsorption on lignin. This helps to explain why simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP), both carried out at 30-32 °C, could offer viable options for mitigating lignin-derived inhibition effects.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zanchetta A,Dos Santos ACF,Ximenes E,da Costa Carreira Nunes C,Boscolo M,Gomes E,Ladisch MRdoi
10.1016/j.biortech.2017.12.061subject
Has Abstractpub_date
2018-03-01 00:00:00pages
143-149eissn
0960-8524issn
1873-2976pii
S0960-8524(17)32204-6journal_volume
252pub_type
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