Abstract:
:Effects of different pulse fed-batch methods on production of the anti-cancer compound 1403C by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403) in a 5-L bioreactor were investigated. Since high glucose concentrations improved mycelial growth but inhibited 1403C production, the cultures were pulse fed with glucose solutions to keep the residual glucose lower than 4 g/L but higher than 0.5 g/L during rapid growth phase (0-50 h). In this way, a maximum dry biomass, 1403C production and yield coefficient (Y1403C/X) of up to 4.5 g/L, 2.64 g/L and 0.59 g/g dry cell weight, respectively were achieved. These values are 22.7%, 98.0% and 61.4%, respectively higher than those obtained with batch cultures. This strategy is valuable for fermentation scale-up of Halorosellinia sp. (No. 1403) for 1403C production, and might also be applicable to other marine fungi cultures.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kang L,Cai M,Yu C,Zhang Y,Zhou Xdoi
10.1016/j.biortech.2011.08.136subject
Has Abstractpub_date
2011-11-01 00:00:00pages
10750-3issue
22eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01284-3journal_volume
102pub_type
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