Abstract：
:A tri-enzyme biocatalyst "combi-CLEAs" with starch hydrolytic activity was prepared from commercially available alpha amylase, glucoamylase and pullulanase preparations by aggregating enzymes with ammonium sulphate followed by cross-linking formed aggregates for 4.5h with 40 mM glutaraldehyde. The effects of precipitant type and cross-linking were studied and the biocatalyst was characterized. Scanning electron microscopy analysis showed that tri-enzyme biocatalyst was of spherical structure. For one pot starch hydrolytic activity, shift in optimum pH from 6 to 7 and temperature from 65 to 75 °C were observed after co-immobilization of enzymes. After one pot starch hydrolysis reaction in batch mode, 100%, 60% and 40% conversions were obtained with combi-CLEAs, separate CLEAs mixture and free enzyme mixture, respectively. Co-immobilization also enhanced the thermal stability of enzymes. Finally, the catalytic activity of enzymes in combi-CLEAs during one pot starch hydrolysis was well maintained up to five cycles without performance changes.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Talekar S,Pandharbale A,Ladole M,Nadar S,Mulla M,Japhalekar K,Pattankude K,Arage Ddoi
10.1016/j.biortech.2013.08.035subject
Has Abstractpub_date
2013-11-01 00:00:00pages
269-275eissn
0960-8524issn
1873-2976pii
S0960-8524(13)01264-9journal_volume
147pub_type
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