Abstract：
:An A. nidulans strain with a pyridoxine marker was used for continuous production of aryl alcohol oxidase (AAO) in a trickle bed reactor (TBR). Modified medium with reduced zinc, no copper, and 5 g/L ascorbic acid that reduced melanin production and increased AAO productivity under growth limited conditions was used. Two air flow rates, 0.11 L/min (0.1 vvm) and 1.1 L/min (1.0 vvm) were tested. More melanin formation and reduced protein productivity were observed with air flow rate of 1.1 L/min. Three random packings were used as support for the fungus inside the TBR column, two of which were hydrophobic and one which was hydrophilic, and three different dilution rates were tested. The use of GEA BCN 030 hydrophobic packing resulted in greater AAO yield and productivity than the other packings. Increasing dilution rates favored melanin formation and citric, lactic and succinic acid accumulation, which decreased AAO yield and productivity.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Pardo-Planas O,Atiyeh HK,Prade RA,Müller M,Wilkins MRdoi
10.1016/j.biortech.2018.01.098subject
Has Abstractpub_date
2018-05-01 00:00:00pages
149-155eissn
0960-8524issn
1873-2976pii
S0960-8524(18)30112-3journal_volume
255pub_type
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