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Enzymatic hydrolysis of chitin pretreated by rapid depressurization from supercritical 1,1,1,2-tetrafluoroethane toward highly acetylated oligosaccharides.

Abstract：

:The hydrolysis of chitin treated under supercritical conditions was successfully carried out using chitinases obtained by an optimized fermentation of the fungus Lecanicillium lecanii. The biopolymer was subjected to a pretreatment based on suspension in supercritical 1,1,1,2-tetrafluoroethane (scR134a), which possesses a critical temperature and pressure of 101°C and 40bar, respectively, followed by rapid depressurization to atmospheric pressure and further fibrillation. This methodology was compared to control untreated chitins and chitin subjected to steam explosion showing improved production of reducing sugars (0.18mg/mL), enzymatic hydrolysis and high acetylation (FA of 0.45) in products with degrees of polymerization between 2 and 5.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Villa-Lerma G,González-Márquez H,Gimeno M,Trombotto S,David L,Ifuku S,Shirai K

doi

10.1016/j.biortech.2016.02.138

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

180-6

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(16)30284-X

journal_volume

209

pub_type

杂志文章

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