Abstract：
:Polyhydroxyalkanoates (PHAs), a family of biodegradable and renewable biopolymers show a huge potential as an alternative to conventional plastics. Extractive bioconversion (in situ product recovery) is a technique that integrates upstream fermentation and downstream purification. In this study, extractive bioconversion of PHAs from Cupriavidus necator H16 was performed via a thermo-separating aqueous two-phase system to reduce the cost and environmental impacts of PHAs production. Key operating parameters, such as polymer concentration, temperature, and pH, were optimized. The strategy achieved a yield and PF of 97.6% and 1.36-fold, respectively at 5% EOPO 3900 concentration, 30 °C fermentation temperature and pH 6. The PHAs production process was also successfully scaled up in a 2 L bioreactor. To the best of our knowledge, this is the first report on extractive fermentation of PHAs from Cupriavidus necator utilizing a thermo-separation system to achieve a better productivity and purity of the target product.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Leong YK,Show PL,Lan JC,Krishnamoorthy R,Chu DT,Nagarajan D,Yen HW,Chang JSdoi
10.1016/j.biortech.2019.121474subject
Has Abstractpub_date
2019-09-01 00:00:00pages
121474eissn
0960-8524issn
1873-2976pii
S0960-8524(19)30704-7journal_volume
287pub_type
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