Abstract：
:Methyl or ethyl esters of long chain fatty acids are called biodiesel. Biodiesel is synthesized by the alcoholysis of oils/fats. In this work, lipase from Thermomyces lanuginosus was precipitated over the clusters of Fe3O4 nanoparticles. This biocatalyst preparation was used for obtaining biodiesel from soybean oil. After optimization of both immobilization conditions and process parameters, complete conversion to biodiesel was obtained in 3h and on lowering the enzyme amount, as little as 1.7U of lipase gave 96% conversion in 7h. The solvent free media with oil:ethanol (w/w) of 1:4 and 40°C with 2% (w/w) water along with 20% (w/w) silica (for facilitating acyl migration) were employed for reaching this high % of conversion. The biocatalyst design enables one to use a rather small amount of lipase. This should help in switching over to a biobased production of biodiesel.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Mukherjee J,Gupta MNdoi
10.1016/j.biortech.2016.02.134subject
Has Abstractpub_date
2016-06-01 00:00:00pages
166-71eissn
0960-8524issn
1873-2976pii
S0960-8524(16)30280-2journal_volume
209pub_type
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