Abstract:
:In this work, a three-level Box-Behnken factorial design was employed combining with response surface methodology (RSM) to optimize the medium composition for the production of the mycelial biomass and exo-polymer in submerged cultures by Grifola frondosa GF9801. A mathematical model was then developed to show the effect of each medium composition and their interactions on the production of mycelial biomass and exo-polymer. The model estimated that, a maximal yield of mycelial biomass (17.61 g/l) could be obtained when the concentrations of glucose, KH2PO4, peptone were set at 45.2 g/l, 2.97 g/l, 6.58 g/l, respectively; while a maximal exo-polymer yield (1.326 g/l) could be achieved when setting concentrations of glucose, KH2PO4, peptone at 58.6 g/l, 4.06 g/l and 3.79 g/l, respectively. These predicted values were also verified by validation experiments. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a significant increase in the yields of mycelial biomass and exo-polymer. Maximum mycelial biomass yield of 22.50 g/l was achieved in a 15-l fermenter using the optimized medium.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Cui FJ,Li Y,Xu ZH,Xu HY,Sun K,Tao WYdoi
10.1016/j.biortech.2005.05.005subject
Has Abstractpub_date
2006-07-01 00:00:00pages
1209-16issue
10eissn
0960-8524issn
1873-2976pii
S0960-8524(05)00255-5journal_volume
97pub_type
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