Abstract：
:A biodegradation facilitator which catalyzes the initial steps of 4-chlorophenol (4-CP) oxidation was prepared by immobilizing multiple enzymes (monooxygenase, CphC-I and dioxygenase, CphA-I) onto a natural inorganic support. The enzymes were obtained via overexpression and purification after cloning the corresponding genes (cphC-I and cphA-I) from Arthrobacter chlorophenolicus A6. Then, the recombinant CphC-I was immobilized onto fulvic acid-activated montmorillonite. The immobilization yield was 60%, and the high enzyme activity (82.6%) was retained after immobilization. Kinetic analysis indicated that the Michaelis-Menten model parameters for the immobilized CphC-I were similar to those for the free enzyme. The enzyme stability was markedly enhanced after immobilization. The immobilized enzyme exhibited a high level of activity even after repetitive use (84.7%) and powdering (65.8%). 4-CP was sequentially oxidized by a multiple enzyme complex, comprising the immobilized CphC-I and CphA-I, via the hydroquinone pathway: oxidative transformation of 4-CP to hydroxyquinol followed by ring fission of hydroxyquinol.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kwean OS,Cho SY,Yang JW,Cho W,Park S,Lim Y,Shin MC,Kim HS,Park J,Kim HSdoi
10.1016/j.biortech.2018.03.066subject
Has Abstractpub_date
2018-07-01 00:00:00pages
268-275eissn
0960-8524issn
1873-2976pii
S0960-8524(18)30414-0journal_volume
259pub_type
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