Abstract：
:Recovery of bioactive compounds, such as proteolytic enzymes, from waste streams is a means to both recuperate value and reduce environmental pollution. Previously optimized lab-scale parameters for the recovery of a stable crude protease fraction from Pacific whiting (Merluccius productus) surimi wash water were tested using pilot plant equipment. Pretreatment of surimi wash water with 60 degrees C heat, acidification to pH 6, and centrifugation doubled ultrafiltration membrane flux and significantly improved protease purity by reducing a majority of the 35-205 kDa proteins. Concentrated crude protease obtained from wash water contained predominantly cathepsin L activity. Enzyme purity was increased about 100-fold, and yield was approximately 80%. Stability (frozen and freeze-dried protease) was maintained for 9 weeks at -80 degrees C. Freeze-dried preparations were also stable for 9 weeks at 4 and -15 degrees C. Successful application of pilot plant conditions allows for sufficient production of protease for further investigations into their applicability.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Mireles DeWitt CA,Morrissey MTdoi
10.1016/s0960-8524(01)00178-xsubject
Has Abstractpub_date
2002-05-01 00:00:00pages
295-301issue
3eissn
0960-8524issn
1873-2976pii
S0960-8524(01)00178-Xjournal_volume
82pub_type
杂志文章abstract：:This study determines cellulose solubilization kinetics from controlled batch digestions and shows the effect of inoculum biomass concentrations. Separate measurements and analyzes were performed for sessile biomass (biofilms) and planktonic biomass (free suspensions). Experiments were conducted using either leachate ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.05.018
更新日期：2009-11-01 00:00:00
abstract：:Pentose-rich hydrolysate obtained from dilute acid pretreatment of oil palm empty fruit bunches was successfully consumed by pentose-consuming yeasts: Cyberlindnera jadinii (Cj) and Pichia jadinii (Pj). Nitrogen supplementation and no additional detoxification step were required. Pj produced 5.87 g/L of biomass using ...
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.03.061
更新日期：2009-09-01 00:00:00
abstract：:Conversion of corn fiber to ethanol in the dry grind process could increase ethanol yields, reduce downstream processing costs and improve overall process profitability. This work investigates the in-situ conversion of corn fiber into ethanol (cellulase addition during simultaneous saccharification and fermentation) d...
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更新日期：2018-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.biortech.2014.11.001
更新日期：2015-01-01 00:00:00
abstract：:Use of lectins as ligands for the immobilization and stabilization of glycoenzymes has immense application in enzyme research and industry. But their widespread use could be limited by the high cost of their production. In the present study preparation of a novel and inexpensive lectin support for use in the immobiliz...
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pub_type： 杂志文章
doi：10.1016/s0960-8524(01)00053-0
更新日期：2001-09-01 00:00:00
abstract：:The production of bioethanol by the conversion of lignocellulosic waste has attracted much interest in recent years because of its low cost and great potential availability. However, the high cost of the enzyme required for this conversion is often considered to be the major bottleneck in the commercial lignocellulosi...
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更新日期：2011-01-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.04.110
更新日期：2015-09-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.05.066
更新日期：2012-08-01 00:00:00
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更新日期：2012-07-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.09.040
更新日期：2010-03-01 00:00:00
abstract：:Anaerobic digestion (AD) of lignocellulosic biomass has received significant attention for bioenergy production in recent years. However, hydrolysis is a rate-limiting in AD of such feedstock. In this study, effects of hydrothermal pretreatment of Napier grass, a model lignocellulosic biomass, on methane yield were ex...
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更新日期：2019-07-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/s0960-8524(01)00006-2
更新日期：2001-06-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.10.023
更新日期：2006-12-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.01.047
更新日期：2010-06-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.10.077
更新日期：2016-01-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.06.019
更新日期：2006-08-01 00:00:00
abstract：:Cinnamomum osmophloeum Kaneh. is an indigenous tree species in Taiwan. In this study, phytochemical characteristics and antioxidant activities of ethanolic extracts from the twigs of C. osmophloeum were investigated for the first time. The extracts were obtained successively using liquid-liquid partition to yield n-he...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.03.020
更新日期：2008-04-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.04.119
更新日期：2018-09-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.10.043
更新日期：2011-02-01 00:00:00
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更新日期：2013-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2009-07-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.06.050
更新日期：2016-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.11.009
更新日期：2005-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
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更新日期：2012-03-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.03.002
更新日期：2015-06-01 00:00:00
abstract：:In this investigation, palm empty fruit bunch (EFB) and almond shell (AS) were implemented as two natural catalysts rich in alkali metals, especially potassium, to enhance the reactivity of tire-char through co-gasification process. Co-gasification experiments were conducted at several blending ratios using isothermal...
journal_title：Bioresource technology
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doi：10.1016/j.biortech.2013.03.179
更新日期：2013-06-01 00:00:00
