Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri.

Abstract:

:Chlorella kessleri accumulates triacylglycerol usable for biodiesel-fuel production to >20% dry cell weight in three days when cultured in three-fold diluted seawater, which imposes the combinatory stress of hyperosmosis and nutrients limitation. The quantitative behavior of major C-compounds, and related-gene expression patterns were investigated in Chlorella cells stressed with hyperosmosis, nutrients limitation, or their combination, to elucidate the C-metabolism for economical seawater-based triacylglycerol accumulation. Combinatory-stress cells showed repressed protein synthesis with initially accumulated starch being degraded later, the C-metabolic flow thereby being diverted to fatty acid and subsequent triacylglycerol accumulation. This C-flow diversion was induced by cooperative actions of nutrients-limitation and hyperosmosis. Semi-quantitative PCR analysis implied positive rewiring of the diverted C-flow into triacylglycerol in combinatory-stress cells through upregulation of gene expression concerning fatty acid and triacylglycerol synthesis, and starch synthesis and degradation. The information of regulatory C-metabolism will help reinforce the seawater-based triacylglycerol accumulation ability in algae including Chlorella.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Otaki R,Oishi Y,Abe S,Fujiwara S,Sato N

doi

10.1016/j.biortech.2019.121686

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

121686

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(19)30916-2

journal_volume

289

pub_type

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