Abstract:
:This study evaluates the effect of sodium (Na(+)) concentration on the growth and PHB production by Cupriavidus necator. Both biomass growth and PHB production were inhibited by Na(+): biomass growth became zero at 8.9 g/L Na(+) concentration while PHB production was completely stopped at 10.5 g/L Na(+). A mathematical model for pure culture heterotrophic PHB production was set up to describe the Na(+) inhibition effect. The parameters related to Na(+) inhibition were estimated based on shake flask experiments. The accumulated Na(+) showed non-linear inhibition effect on biomass growth but linear inhibition effect on PHB production kinetics. Fed-batch experiments revealed that a high accumulation of Na(+) due to a prolonged growth phase, using NaOH for pH control, decreased the subsequent PHB production. The model was validated based on independent experimental data sets, showing a good agreement between experimental data and simulation results.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Mozumder MS,Garcia-Gonzalez L,De Wever H,Volcke EIdoi
10.1016/j.biortech.2015.04.110subject
Has Abstractpub_date
2015-09-01 00:00:00pages
213-8eissn
0960-8524issn
1873-2976pii
S0960-8524(15)00636-7journal_volume
191pub_type
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