Effect of sodium accumulation on heterotrophic growth and polyhydroxybutyrate (PHB) production by Cupriavidus necator.

Abstract:

:This study evaluates the effect of sodium (Na(+)) concentration on the growth and PHB production by Cupriavidus necator. Both biomass growth and PHB production were inhibited by Na(+): biomass growth became zero at 8.9 g/L Na(+) concentration while PHB production was completely stopped at 10.5 g/L Na(+). A mathematical model for pure culture heterotrophic PHB production was set up to describe the Na(+) inhibition effect. The parameters related to Na(+) inhibition were estimated based on shake flask experiments. The accumulated Na(+) showed non-linear inhibition effect on biomass growth but linear inhibition effect on PHB production kinetics. Fed-batch experiments revealed that a high accumulation of Na(+) due to a prolonged growth phase, using NaOH for pH control, decreased the subsequent PHB production. The model was validated based on independent experimental data sets, showing a good agreement between experimental data and simulation results.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Mozumder MS,Garcia-Gonzalez L,De Wever H,Volcke EI

doi

10.1016/j.biortech.2015.04.110

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

213-8

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(15)00636-7

journal_volume

191

pub_type

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