Abstract:
:Effects of substrate-selective inoculum prepared by growing on glucose, xylose, arabinose, GXA (glucose, xylose, arabinose, 1:1:1) and corn stover hydrolyzate (dilute acid pretreated and enzymatically hydrolyzed, CSH) on ethanol production from CSH by a mixed sugar utilizing recombinant Escherichia coli (strain FBR5) were investigated. The initial ethanol productivity was faster for the seed grown on xylose followed by GXA, CSH, glucose and arabinose. Arabinose grown seed took the longest time to complete the fermentation. Delayed saccharifying enzyme addition in simultaneous saccharification and fermentation of dilute acid pretreated CS by the recombinant E. coli strain FBR5 allowed the fermentation to finish in a shorter time than adding the enzyme simultaneously with xylose grown inoculum. Use of substrate selective inoculum and fermenting pentose sugars first under glucose limited condition helped to alleviate the catabolite repression of the recombinant bacterium on ethanol production from lignocellulosic hydrolyzate.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Saha BC,Qureshi N,Kennedy GJ,Cotta MAdoi
10.1016/j.biortech.2015.04.079subject
Has Abstractpub_date
2015-08-01 00:00:00pages
182-8eissn
0960-8524issn
1873-2976pii
S0960-8524(15)00605-7journal_volume
190pub_type
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