Eukaryotic and prokaryotic microbial communities during microalgal biomass production.

Abstract:

:Eukaryotic and bacterial communities were characterized and quantified in microalgal photobioreactor cultures of freshwater Chlorella vulgaris and marine Dunaliella tertiolecta. The microalgae exhibited good growth, whilst both cultures contained diverse bacterial communities. Both cultures included Proteobacteria and Bacteroidetes, while C. vulgaris cultures also contained Actinobacteria. The bacterial genera present in the cultures were different due to different growth medium salinities and possibly different extracellular products. Bacterial community profiles were relatively stable in D. tertiolecta cultures but not in C. vulgaris cultures likely due to presence of ciliates (Colpoda sp.) in the latter. The presence of ciliates did not, however, cause decrease in total number of C. vulgaris or bacteria during 14 days of cultivation. Quantitative PCR (qPCR) reliably showed relative microalgal and bacterial cell numbers in the batch cultures with stable microbial communities, but was not effective when bacterial communities varied. Raw culture samples were successfully used as qPCR templates.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Lakaniemi AM,Hulatt CJ,Wakeman KD,Thomas DN,Puhakka JA

doi

10.1016/j.biortech.2012.08.048

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

387-93

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)01227-8

journal_volume

124

pub_type

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