Abstract:
:Eukaryotic and bacterial communities were characterized and quantified in microalgal photobioreactor cultures of freshwater Chlorella vulgaris and marine Dunaliella tertiolecta. The microalgae exhibited good growth, whilst both cultures contained diverse bacterial communities. Both cultures included Proteobacteria and Bacteroidetes, while C. vulgaris cultures also contained Actinobacteria. The bacterial genera present in the cultures were different due to different growth medium salinities and possibly different extracellular products. Bacterial community profiles were relatively stable in D. tertiolecta cultures but not in C. vulgaris cultures likely due to presence of ciliates (Colpoda sp.) in the latter. The presence of ciliates did not, however, cause decrease in total number of C. vulgaris or bacteria during 14 days of cultivation. Quantitative PCR (qPCR) reliably showed relative microalgal and bacterial cell numbers in the batch cultures with stable microbial communities, but was not effective when bacterial communities varied. Raw culture samples were successfully used as qPCR templates.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Lakaniemi AM,Hulatt CJ,Wakeman KD,Thomas DN,Puhakka JAdoi
10.1016/j.biortech.2012.08.048subject
Has Abstractpub_date
2012-11-01 00:00:00pages
387-93eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01227-8journal_volume
124pub_type
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