当前位置： SCI文献检索 > BIORESOURCE TECHNOLOGY期刊下所有文献 > Expression, purification and use of the soluble domain of Lactobacillus paracasei beta-fructosidase to optimise production of bioethanol from grass fructans.

Expression, purification and use of the soluble domain of Lactobacillus paracasei beta-fructosidase to optimise production of bioethanol from grass fructans.

Abstract：

:Microbial inulinases find application in food, pharmaceutical and biofuel industries. Here, a novel Lactobacillus paracasei beta-fructosidase was overexpressed as truncated cytosolic protein ((t)fosEp) in Escherichia coli. Purified (t)fosEp was thermostable (10-50 degrees C) with a pH optimum of 5; it showed highest affinity for bacterial levan (beta[2-6] linked fructose) followed by nystose, chicory inulin, 1-kestose (beta[2-1] linkages) and sucrose (K(m) values of 0.5, 15, 15.6, 49 and 398 mM, respectively). Hydrolysis of polyfructose moieties in agriculturally-sourced grass juice (GJ) with (t)fosEp resulted in the release of >13 mg/ml more bioavailable fructose than was measured in untreated GJ. Bioethanol yields from fermentation experiments with Brewer's yeast and GJ+(t)fosEp were >25% higher than those achieved using untreated GJ feedstock (36.5[+/-4.3] and 28.2[+/-2.7]mg ethanol/ml, respectively). This constitutes the first specific study of the potential to ferment ethanol from grass juice and the utility of a novel core domain of beta-fructosidase from L. paracasei.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Martel CM,Warrilow AG,Jackson CJ,Mullins JG,Togawa RC,Parker JE,Morris MS,Donnison IS,Kelly DE,Kelly SL

doi

10.1016/j.biortech.2010.01.084

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

4395-402

issue

12

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)00170-7

journal_volume

101

pub_type

杂志文章

相关文献

BIORESOURCE TECHNOLOGY文献大全
  • Recent advances in alcohol and organic acid fractionation of lignocellulosic biomass.

    abstract：:Organosolv fractionation is a promising process to separate lignocellulosic biomass for the preparation of multiply products including biofuels, chemicals, and materials. This review presents the state of art of different processes applying alcohols and organic acids to treat lignocellulosic biomass for the production...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/j.biortech.2015.10.004

    authors： Li MF,Yang S,Sun RC

    更新日期：2016-01-01 00:00:00

  • Bacterial transformation and biodegradation processes simulation in horizontal subsurface flow constructed wetlands using CWM1-RETRASO.

    abstract：:The performance and reliability of the CWM1-RETRASO model for simulating processes in horizontal subsurface flow constructed wetlands (HSSF CWs) and the relative contribution of different microbial reactions to organic matter (COD) removal in a HSSF CW treating urban wastewater were evaluated. Various different approa...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2010.09.038

    authors： Llorens E,Saaltink MW,Poch M,García J

    更新日期：2011-01-01 00:00:00

  • Synergetic promotion of direct interspecies electron transfer for syntrophic metabolism of propionate and butyrate with graphite felt in anaerobic digestion.

    abstract：:Direct interspecies electron transfer (DIET) was considered to replace interspecies H2 transfer to enhance syntrophic metabolism during the anaerobic digestion. The graphite felt with excellent electrical conductivity was added into a semi-continuous reactor as a conductive material, strengthening DIET in enhancing th...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.121373

    authors： Zhang M,Ma Y,Ji D,Li X,Zhang J,Zang L

    更新日期：2019-09-01 00:00:00

  • Development of natural cellulase inhibitor mediated intensified biological pretreatment technology using Pleurotus florida for maximum recovery of cellulose from paddy straw under solid state condition.

    abstract：:Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignificati...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.07.105

    authors： Naresh Kumar M,Ravikumar R,Thenmozhi S,Kirupa Sankar M

    更新日期：2017-11-01 00:00:00

  • Process performance and modelling of anaerobic digestion using source-sorted organic household waste.

    abstract：:Three distinctive start-up strategies of biogas reactors fed with source-sorted organic fraction of municipal solid waste were investigated to reveal the most reliable procedure for rapid process stabilization. Moreover, the experimental results were compared with mathematical modeling outputs. The initial inoculation...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.09.122

    authors： Khoshnevisan B,Tsapekos P,Alvarado-Morales M,Angelidaki I

    更新日期：2018-01-01 00:00:00

  • Solids removal in upflow anaerobic reactors, a review.

    abstract：:This desk study deals with the mechanisms and parameters affecting particles separation from wastewater in mainly upflow anaerobic reactors. Despite the fact that the functioning of upflow anaerobic sludge blanket (UASB) systems depends on both physical parameters and biological processes, the physical parameters have...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/s0960-8524(03)00095-6

    authors： Mahmoud N,Zeeman G,Gijzen H,Lettinga G

    更新日期：2003-10-01 00:00:00

  • Protease increases fermentation rate and ethanol yield in dry-grind ethanol production.

    abstract：:The effects of acid protease and urea addition during the fermentation step were evaluated. The fermentations were also tested with and without the addition of urea to determine if protease altered the nitrogen requirements of the yeast. Results show that the addition of the protease had a statistically significant ef...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2013.11.043

    authors： Johnston DB,McAloon AJ

    更新日期：2014-02-01 00:00:00

  • Optimization of Spongiochloris sp. biomass production in the abattoir digestate.

    abstract：:In the present study, the abattoir digestate was used as a culture medium for Spongiochloris sp. growth with added mineral components under optimized conditions in batch culture. Firstly, an Hadamard matrix was used to investigate the impact of certain influencing factors on the Spongiochloris sp. growth. Then, a frac...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2010.11.036

    authors： Bchir FS,Gannoun H,El Herry S,Hamdi M

    更新日期：2011-02-01 00:00:00

  • Enhanced biomethanation of kitchen waste by different pre-treatments.

    abstract：:Five different pre-treatments were investigated to enhance the solubilisation and anaerobic biodegradability of kitchen waste (KW) in thermophilic batch and continuous tests. In the batch solubilisation tests, the highest and the lowest solubilisation efficiency were achieved with the thermo-acid and the pressure-depr...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2010.07.122

    authors： Ma J,Duong TH,Smits M,Verstraete W,Carballa M

    更新日期：2011-01-01 00:00:00

  • Simultaneous improving nitrogen removal and decreasing greenhouse gas emission with biofilm carriers addition in ecological floating bed.

    abstract：:Ecological floating bed (EFB) is a green technology for treatment of micro-polluted wastewater. However, its nitrogen removal efficiency is still unsatisfactory. In this study, two EFBs with additional carbon source were established to explore biofilm carriers addition on nitrogen removal and greenhouse gas (GHG) emis...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/j.biortech.2019.121944

    authors： Sun S,Liu J,Zhang M,He S

    更新日期：2019-11-01 00:00:00

  • Formation and use of anaerobic consortia for the biotransformation of sulfur-containing extracts from pre-oxidized crude oil and oil fractions.

    abstract：:A new solution for fossil raw materials desulfurization based on a hybrid chemical-biocatalytic scheme with biogas and sulfide production is proposed.·H2O2, formic acid and Na2MoO4 were used for petroleum or oil fractions pre-oxidation. Ethanol or dimethylformamide was used as extractant to remove sulfur-contained com...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2020.124248

    authors： Maslova O,Senko O,Stepanov N,Gladchenko M,Gaydamaka S,Akopyan A,Polikarpova P,Lysenko S,Anisimov A,Efremenko E

    更新日期：2021-01-01 00:00:00

  • Bio-utilization of cheese manufacturing wastes (cheese whey powder) for bioethanol and specific product (galactonic acid) production via a two-step bioprocess.

    abstract：:Cheese whey, produced from coagulation of milk during cheese manufacture, is a major environmental pollutant. The most abundant component in cheese whey is lactose, which is a potential resource for various value-added chemicals. Here, a two-step bioprocess using Saccharomyces cerevisiae and Gluconobacter oxydans to b...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2018.10.001

    authors： Zhou X,Hua X,Huang L,Xu Y

    更新日期：2019-01-01 00:00:00

  • Comparative study of activated sludge with different individual nitrogen sources at a low temperature: Effluent dissolved organic nitrogen compositions, metagenomic and microbial community.

    abstract：:The objective of this study was to explore nitrogen removal, especially effluent dissolved organic nitrogen (DON) composition, relative genes and microbial community structures with four individual nitrogen sources at 5°C. Results show that effluent DON did not have dependent relationship with the TN removal rate (ure...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.09.026

    authors： He S,Ding L,Li K,Hu H,Ye L,Ren H

    更新日期：2018-01-01 00:00:00

  • Improved production of the anticancer compound 1403C by glucose pulse feeding of marine Halorosellinia sp. (No. 1403) in submerged culture.

    abstract：:Effects of different pulse fed-batch methods on production of the anti-cancer compound 1403C by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403) in a 5-L bioreactor were investigated. Since high glucose concentrations improved mycelial growth but inhibited 1403C production, the cultures were pulse fed w...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2011.08.136

    authors： Kang L,Cai M,Yu C,Zhang Y,Zhou X

    更新日期：2011-11-01 00:00:00

  • Consolidated bioprocesses for efficient bioconversion of palm biomass wastes into biodiesel feedstocks by oleaginous fungi and yeasts.

    abstract：:Consolidated bioprocesses for bioconversion of lignocellulosic biomass into biodiesel feedstocks were developed. Palm empty fruit bunch (EFB) was biologically pretreated coupling with fungal lipid production (121.4 ± 2.7 mg/g-EFB) by lignocellulolytic oleaginous fungi prior to lipid production by oleaginous yeasts. In...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2020.123893

    authors： Intasit R,Cheirsilp B,Louhasakul Y,Boonsawang P

    更新日期：2020-11-01 00:00:00

  • Enhanced bioelectricity generation by improving pyocyanin production and membrane permeability through sophorolipid addition in Pseudomonas aeruginosa-inoculated microbial fuel cells.

    abstract：:Improvement on electron shuttle-mediated extracellular electron transfer (EET) is of great potential to enhance the power output of MFCs. In this study, sophorolipid was added to enhance the performance of Pseudomonas aeruginosa-inoculated MFC by improving the electron shuttle-mediated EET. Upon sophorolipid addition,...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2014.05.093

    authors： Shen HB,Yong XY,Chen YL,Liao ZH,Si RW,Zhou J,Wang SY,Yong YC,OuYang PK,Zheng T

    更新日期：2014-09-01 00:00:00

  • Comparison of stirred tank and airlift bioreactors in the production of polygalacturonases by Aspergillus oryzae.

    abstract：:The production of endo and exo-polygalacturonase (PG) by Aspergillus oryzae IPT 301 was studied in a stirred tank bioreactor (STR) and an internal circulation airlift bioreactor. Using a factorial experimental design, a soluble culture medium was defined which allowed the production of exo- and endo-PG comparable to t...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2008.11.062

    authors： Fontana RC,Polidoro TA,da Silveira MM

    更新日期：2009-10-01 00:00:00

  • Biorefinery process for production of paper and oligomers from Leucaena leucocephala K360 with or without prior autohydrolysis.

    abstract：:Lignocellulosic material from Leucaena leucocephala was subjected to a two-stage fractionation process to obtain a valorized effluent containing hemicellulose derivatives and a solid phase for producing cellulose pulp by conventional soda-anthraquinone delignification. This solid phase allows the production of cellulo...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.09.051

    authors： Feria MJ,García JC,Díaz MJ,Fernández M,López F

    更新日期：2012-12-01 00:00:00

  • Oxidation of glycerol by 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) in the presence of laccase.

    abstract：:Glycerol has the potential of being a low-cost and extremely versatile building block. However, current transformation strategies such based on noble-metal-catalysts show several disadvantages including catalyst deactivation or negative environmental impacts. In this study glycerol was oxidized by 2,2,6,6-tetramethylp...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2009.04.051

    authors： Liebminger S,Siebenhofer M,Guebitz G

    更新日期：2009-10-01 00:00:00

  • Microwave-enhanced pyrolysis of macroalgae and microalgae for syngas production.

    abstract：:In this study, three different marine biomasses, i.e., microalgae-spirulina, chlorella and macroalgae-porphyra, were pyrolyzed in a laboratory-scale multimode-microwave cavity at 400, 550 and 700°C. Ovalbumin and cellulose were also chosen as model compounds to simulate algae. The influence of heating rate on pyrolysi...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.02.006

    authors： Hong Y,Chen W,Luo X,Pang C,Lester E,Wu T

    更新日期：2017-08-01 00:00:00

  • Influence of carbon and buffer amendment on ammonia volatilization in composting.

    abstract：:Laboratory-scale experiments were carried out to test a mathematical model of the nitrogen dynamics in a composting process. The main ingredients of composting materials were wheat straw and dairy manure. The influence of (a) two carbon amendments, i.e. molasses and office paper, and (b) two chemicals forming buffer s...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2005.03.041

    authors： Liang Y,Leonard JJ,Feddes JJ,McGill WB

    更新日期：2006-03-01 00:00:00

  • Examining structure-activity correlations of some high activity enzyme preparations for low water media.

    abstract：:A first study of the comparison of structures of enzymes (by FT-IR and CD) in different high activity (in low water media) preparations is reported. Using chymotrypsin and subtilisin as models, we have studied various factors that distinguish enzyme precipitated and rinsed with propanol (EPRP), crosslinked enzyme aggr...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2011.12.066

    authors： Solanki K,Gupta MN,Halling PJ

    更新日期：2012-07-01 00:00:00

  • Recycling of wood chips and wheat dregs for sludge processing.

    abstract：:A Buchner filtration study was conducted to investigate the effect on sludge dewatering of adding organic waste solids (wood chips or wheat dregs) to sludge after chemical preconditioning (with ferric chloride or alum). Increasing the dose of wood chips or wheat dregs enhanced sludge filtration performance and increas...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/s0960-8524(00)00098-5

    authors： Lin YF,Jing SR,Lee DY

    更新日期：2001-01-01 00:00:00

  • Bioconversion of barley straw lignin into biodiesel using Rhodococcus sp. YHY01.

    abstract：:Rhodococcus sp. YHY01 was studied to utilize various lignin derived aromatic compounds. It was able to utilize p-coumaric acid, cresol, and 2,6 dimethoxyphenol and resulted in biomass production i.e. 0.38 g dcw/L, 0.25 g dcw/L and 0.1 g dcw/L, and lipid accumulation i.e. 49%, 40%, 30%, respectively. The half maximal i...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.121704

    authors： Bhatia SK,Gurav R,Choi TR,Han YH,Park YL,Park JY,Jung HR,Yang SY,Song HS,Kim SH,Choi KY,Yang YH

    更新日期：2019-10-01 00:00:00

  • Distribution of heavy metals and hydrocarbon contents in an alfisol contaminated with waste-lubricating oil amended with organic wastes.

    abstract：:Contamination of soil and groundwater with mineral oil-based products is among the most common sources of pollution in Nigeria. This study evaluated the distribution of some heavy metals and hydrocarbon content in soil contaminated with waste-lubricating oil (spent oil), and the effectiveness of some abundantly availa...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2007.05.048

    authors： Adesodun JK,Mbagwu JS

    更新日期：2008-05-01 00:00:00

  • Biosynthesis of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate by carbonyl reductase from Rhodosporidium toruloides in mono and biphasic media.

    abstract：:tert-Butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate ((3R,5S)-CDHH) is the key intermediate for synthesis of atorvastatin and rosuvastatin. Carbonyl reductase exhibits excellent activity toward tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate ((S)-CHOH) to synthesize (3R,5S)-CDHH. In this study, a whole cell biosynthesi...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.09.204

    authors： Liu ZQ,Wu L,Zheng L,Wang WZ,Zhang XJ,Jin LQ,Zheng YG

    更新日期：2018-02-01 00:00:00

  • Performance of subsurface flow constructed wetland taking pretreated swine effluent under heavy loads.

    abstract：:Subsurface flow constructed wetlands (SSFCW) subjected to changing of loading rates are poorly understood, especially when used to treat swine waste under heavy loads. This study employed a SSFCW system to take pretreated swine effluent at three hydraulic retention times (HRT): 8.5-day HRT (Phase I), 4.3-day HRT (Phas...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2003.08.012

    authors： Lee CY,Lee CC,Lee FY,Tseng SK,Liao CJ

    更新日期：2004-04-01 00:00:00

  • Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization.

    abstract：:In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2021.124673

    authors： Saratale RG,Cho SK,Saratale GD,Ghodake GS,Bharagava RN,Kim DS,Nair S,Shin HS

    更新日期：2021-03-01 00:00:00

  • Bioethanol fermentation of concentrated rice straw hydrolysate using co-culture of Saccharomyces cerevisiae and Pichia stipitis.

    abstract：:Rice straw is one of the abundant lignocellulosic feed stocks in the world and has been selected for producing ethanol at an economically feasible manner. It contains a mixture of sugars (hexoses and pentoses). Biphasic acid hydrolysis was carried out with sulphuric acid using rice straw. After acid hydrolysis, the su...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2011.03.019

    authors： Yadav KS,Naseeruddin S,Prashanthi GS,Sateesh L,Rao LV

    更新日期：2011-06-01 00:00:00

  • Efficient azo dye decolorization in a continuous stirred tank reactor (CSTR) with built-in bioelectrochemical system.

    abstract：:A continuous stirred tank reactor with built-in bioelectrochemical system (CSTR-BES) was developed for azo dye Alizarin Yellow R (AYR) containing wastewater treatment. The decolorization efficiency (DE) of the CSTR-BES was 97.04±0.06% for 7h with sludge concentration of 3000mg/L and initial AYR concentration of 100mg/...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2016.07.135

    authors： Cui MH,Cui D,Gao L,Cheng HY,Wang AJ

    更新日期：2016-10-01 00:00:00