Abstract：
:A first study of the comparison of structures of enzymes (by FT-IR and CD) in different high activity (in low water media) preparations is reported. Using chymotrypsin and subtilisin as models, we have studied various factors that distinguish enzyme precipitated and rinsed with propanol (EPRP), crosslinked enzyme aggregates (CLEA), protein coated microcrystals (PCMC) and crosslinked protein coated microcrystals (CLPCMC). The suspensions in organic media were assayed for catalytic activity, and structures were probed by FT-IR and CD measurements. CD studies of enzyme suspensions were possible by using a rotating cell accessory. There was a generally good correlation between higher catalytic activity and retention of native structures. Activity and retention of native structure was always higher if aqueous enzyme solution was added to propanol rather than vice versa in the precipitation step of these preparations. The work identifies factors which may lead to better biocatalyst designs for low water media.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Solanki K,Gupta MN,Halling PJdoi
10.1016/j.biortech.2011.12.066subject
Has Abstractpub_date
2012-07-01 00:00:00pages
147-51eissn
0960-8524issn
1873-2976pii
S0960-8524(11)01812-8journal_volume
115pub_type
杂志文章abstract：:This study prepared a carboxylic functionalized bioadsorbent that met the "4-E" criteria: Efficient, Economical, Environmentally friendly, and Easily-produced. Sesame straw (Sesamum indicum L.) was functionalized through treatment with citric acid (SSCA) and tartaric acid (SSTA). The products were examined for adsorpt...
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abstract：:Biofiltration is the most commonly used biological gas treatment technology and is extensively used for the treatment of polluted air with gas flow rates of up to 2 x 10(5) m(3)/h. It involves a filter bed of organic matter serving both as carrier for microorganisms and as nutrient supplier. Polluted gas passes throug...
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abstract：:The biosorption of Hg(2+) by two strains of cyanobacteria, Spirulina platensis and Aphanothece flocculosa, was studied under a batch stirred reaction system. Essential process parameters, including pH, biomass concentration, initial metal concentration, and presence of co-ions were shown to influence the Hg(2+) uptake...
journal_title：Bioresource technology
pub_type： 杂志文章
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更新日期：2008-09-01 00:00:00
abstract：:Activated carbon was prepared from an inexpensive and renewable carbon source, Typha orientalis, by H(3)PO(4) activation and then impregnated with different Mn salts and tested for its Neutral Red (NR) adsorption capacities. The amount of Mn impregnated in the activated carbon was influenced by the anion species. Impr...
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journal_title：Bioresource technology
pub_type： 杂志文章,评审
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更新日期：2012-06-01 00:00:00
abstract：:A solvent tolerant strain of Pseudomonas aeruginosa (PseA) was isolated from soil samples by cyclohexane enrichment in medium. The strain was able to sustain and grow in a wide range of organic solvents. The adaptation of P. aeruginosa cell towards solvents was seen at membrane level in transmission electron micrograp...
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.09.064
更新日期：2011-12-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.08.082
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abstract：:InCl3, a water-compatible Lewis acid, was used for the conversion of microcrystalline cellulose to produce 5-hydroxymethylfurfural (HMF) in a H2O/THF biphasic system. Addition of NaCl increased the HMF yield significantly but suppressed the levulinic acid (LA) formation. The HMF yield of 39.7% was obtained in 2h at 20...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.09.077
更新日期：2014-11-01 00:00:00
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abstract：:A novel stepwise pretreatment of combination of fungal treatment with liquid hot water (LHW) treatment was conducted to enhance the enzymatic hydrolysis of Populus tomentosa. The results showed that lignin and cellulose increased with the elevating temperature, while significant amount of hemicellulose was degraded du...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.12.116
更新日期：2012-03-01 00:00:00
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doi：10.1016/j.biortech.2010.12.109
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pub_type： 杂志文章,评审
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更新日期：2018-04-01 00:00:00
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更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2013-09-01 00:00:00
abstract：:The aim of this study was to investigate the Cr(VI) biosorption potential of immobilized Rhizopus nigricans and to screen a variety of non-toxic desorbing agents, in order to find out possible application in multiple sorption-desorption cycles. The biomass was immobilized by various mechanisms and evaluated for remova...
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更新日期：2003-03-01 00:00:00
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abstract：:Oil palm empty fruit bunch pellets were subjected to pyrolysis in a multimode microwave (MW) system (1 kW and 2.45 GHz frequency) with and without the MW absorber, activated carbon. The ratio of biomass to MW absorber not only affected the temperature profiles of the EFB but also pyrolysis products such as bio-oil, ch...
journal_title：Bioresource technology
pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
abstract：:The anaerobic digestion model No. 1 (ADM1), conceived by the international water association (IWA) task group for mathematical modelling of anaerobic digestion processes is a structured generic model which includes multiples steps describing biochemical and physicochemical processes encountered in the anaerobic degrad...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.11.035
更新日期：2008-09-01 00:00:00
abstract：:Physicochemical evolution (i.e. pore structure variation, carbon structure change and active AAEM transformation) during rice straw (RS) and Shenfu bituminous coal (SF) co-pyrolysis was quantitatively determined in this work. Moreover, the corresponding char gasification was conducted using a thermogravimetric analyze...
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