Abstract:
:Lipid extraction directly from the wet oleaginous microorganisms for biodiesel production is preferred as it reduces the energy input for traditional processes which require extensive drying of the biomass prior to the extraction. The high water content (≥80% on cell dry weight) in the wet biomass hinders the extraction efficiency due to the mass transfer limitation. This limitation can be overcome by pretreating wet biomass prior to the lipid extraction using pressurized gas that can be used alone or combined with other pretreatments to disrupt the cell wall. In this review, an extensive discussion on different pretreatments and the subsequent lipid extraction using these pretreatments is presented. Furthermore, a detailed account of the cell disruption using pressurized gas (e.g., CO2) treatment for microbial cell lysing is also presented. Finally, a new technique on lipid extraction directly from wet biomass using the combination of pressurized CO2 and microwave pretreatment is proposed.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Howlader MS,Rai N,Todd French Wdoi
10.1016/j.biortech.2018.07.092subject
Has Abstractpub_date
2018-11-01 00:00:00pages
743-755eissn
0960-8524issn
1873-2976pii
S0960-8524(18)31021-6journal_volume
267pub_type
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