A protease stable in organic solvents from solvent tolerant strain of Pseudomonas aeruginosa.

Abstract:

:A solvent tolerant strain of Pseudomonas aeruginosa (PseA) was isolated from soil samples by cyclohexane enrichment in medium. The strain was able to sustain and grow in a wide range of organic solvents. The adaptation of P. aeruginosa cell towards solvents was seen at membrane level in transmission electron micrographs. It also secreted a novel protease, which exhibited remarkable solvent stability and retained most of the activity at least up to 10 days in the presence of hydrophobic organic solvents (log P > or = 2.0) at 25% (v/v) concentrations. The protease was able to withstand as high as 75% concentration of solvents at least up to 48 h. P. aeruginosa strain and its protease, both seem promising for solvent bioremediation, wastewater treatment and carrying out biotransformation in non-aqueous medium.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Gupta A,Khare SK

doi

10.1016/j.biortech.2005.09.006

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

1788-93

issue

15

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(05)00431-1

journal_volume

97

pub_type

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