Abstract：
:The major purpose of this study was to investigate the effects of operational factors and bioprocess strategies on the mixotrophic cultivation of a microalgae Chlorella sorokiniana MB-1 for lutein production. Aeration with CO2 showed the highest biomass productivity and lutein productivity of 0.89 g/L/d and 3.49 mg/L/d, respectively. Semi-batch operation performed with 80% medium replacement ratio resulted in the highest biomass productivity and lutein productivity of 1.55 g/L/d and 5.51 mg/L/d, respectively. A two-stage strategy was developed to enhance the biomass production of the MB-1 strain in stage 1 with semi-batch mixotrophic culture and to optimize lutein accumulation in stage 2 under photoautotrophic conditions. The maximum biomass productivity and lutein productivity was 1.98 g/L/d and 7.62 mg/L/d, respectively, with a medium replacement ratio of 80% in stage 1. Compared with batch cultivation, the lutein productivity was enhanced by 32.7% for semi-batch operation alone and 85.9% for the semi-batch-integrated two-stage process.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Chen CY,Liu CCdoi
10.1016/j.biortech.2018.04.024subject
Has Abstractpub_date
2018-08-01 00:00:00pages
74-79eissn
0960-8524issn
1873-2976pii
S0960-8524(18)30537-6journal_volume
262pub_type
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